Direct In vitro Regeneration of Sugarcane Var. China (Saccharum officinarum L.) Using Leaf-Sheath Explants

Authors

  • M H Kabir Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Bangladesh Atomic Energy Commission, Post: DEPZ-1349, Savar, Dhaka, Bangladesh
  • Pronabananda Das Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Bangladesh Atomic Energy Commission, Post: DEPZ-1349, Savar, Dhaka, Bangladesh
  • Md Monirul Islam Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Bangladesh Atomic Energy Commission, Post: DEPZ-1349, Savar, Dhaka, Bangladesh
  • A N K Mamun Plant Biotechnology and Genetic Engineering Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Bangladesh Atomic Energy Commission, Post: DEPZ-1349, Savar, Dhaka, Bangladesh

DOI:

https://doi.org/10.3329/ptcb.v34i2.78906

Keywords:

Direct plant regeneration, In vitro, Leaf sheath explants, Sugarcane, MS

Abstract

An efficient and reproducible in vitro regeneration protocol was developed for sugarcane var. China using leaf-sheath explants. Direct multiple shoot formation without the intervention of callus from leaf-sheath was achieved on MS medium supplemented with 10.0 mg/l NAA. Ninety percent of the explants were found to induce shoots following a 90-day culture period. After 90 days of cultivation, each explant produced an average of 30.0 ± 2.50 shoots having a mean shoot length of 7.2 ± 0.50 cm on this medium. Within 30 days of being exposed to 1/2 strength of MS containing 2.50 mg/l NAA, 90% of the regenerated shoots produced roots. The average root length in this medium was 7.50 ± 2.30 cm, and there were 15.0 ± 1.25 roots produced from each shoot. Ninety percent of the in vitro raised plantlets were found to survive in the soil under a natural environment.

Plant Tissue Cult. & Biotech. 34(2): 115-121, 2024 (December)

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Published

2025-01-07

How to Cite

Kabir, M. H., Das, P., Islam , M. M., & Mamun, A. N. K. (2025). Direct In vitro Regeneration of Sugarcane Var. China (Saccharum officinarum L.) Using Leaf-Sheath Explants. Plant Tissue Culture and Biotechnology, 34(2), 115–121. https://doi.org/10.3329/ptcb.v34i2.78906

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