Screening of Wheat Varieties and Advanced Lines for Salinity Tolerance at the Seedling Stage through Morpho-Molecular Approaches

Authors

  • Md Sajibur Rahman Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • AHM Osama Haque Department of Computer Science, United International University, United City, Madani Avenue, Badda, Dhaka, Dhaka-1212, Bangladesh.
  • Surya Afrin Shorna Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Sadia Jafrin Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Md Shadman Sakib Mim Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • Mirza Mofazzal Islam Breeding Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh-2202, Bangladesh.
  • Md Shahidul Haque Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

DOI:

https://doi.org/10.3329/ptcb.v34i2.78900

Keywords:

Bread wheat, Hydroponic culture, Salt stress, Selection, SSR marker

Abstract

The selection of potential salinity tolerant varieties is important for the cultivation of wheat in saline prone areas. A screening was performed to assess the salt tolerance capacity of 15 wheat varieties of Bangladesh and 10 advanced lines (exotic) in a hydroponic culture system at four distinct salt concentrations (0, 12, 16, and 20 dS/m). The results revealed that different salinity levels significantly affect the growth attributes by reducing the shoot length and fresh as well as dry weight of roots and shoots, with a few exceptions in some genotypes at 12 dS/m salinity. The highest STI (Salt tolerance index) was observed in nine genotypes, namely BINA Gom-1, ESWYT P-44, ESWYT P-28, BARI Gom-23, ESWYT P-19, BARI Gom-27, BARI Gom-29, Pavon-76 and BARI Gom-32 which are regarded as tolerant varieties and advanced lines. The wheat genotypes were subjected to molecular assessment using 21 Single Sequence Repeat (SSR) markers associated with salinity tolerance. SSR marker assisted assessment identified 116 alleles in 25 wheat genotypes, with an average of 5.52 alleles per locus. In this experiment, the marker Xwmc-24 generated the highest (0.825) polymorphism information content (PIC) and Nei's (1973) gene diversity (0.845). Twenty-five genotypes were categorized into six distinct clusters using similarity indices-based cluster analysis. Advanced lines, namely ESWYT P-44, ESWYT P-28, ESWYT P-19 and HYVs, namely BARI Gom-27 and BARI Gom-29, were grouped in cluster 5, while BINA Gom-1 was identified in cluster 1. Considering all the facts, it can be concluded that these just mentioned varieties and inbred advanced lines may be the potential candidates for breeding programs towards salinity tolerance in wheat.

Plant Tissue Cult. & Biotech. 34(2): 173-191, 2024 (December)

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Published

2025-01-07

How to Cite

Rahman, M. S., Haque, A. O., Shorna, S. A., Jafrin, S., Mim, M. S. S., Islam, M. M., & Haque, M. S. (2025). Screening of Wheat Varieties and Advanced Lines for Salinity Tolerance at the Seedling Stage through Morpho-Molecular Approaches. Plant Tissue Culture and Biotechnology, 34(2), 173–191. https://doi.org/10.3329/ptcb.v34i2.78900

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