Optimization of Plant Growth Regulators for Efficient Embryogenic Callus Induction and Subsequent Plant Regeneration in Two Indigenous Aromatic Rice Varieties of Bangladesh

Authors

  • Mimma Afrin Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh.
  • Sium Ahmed Cell Genetics and Plant Biotechnology Laboratory (CGPBL), Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh
  • Tanvir Ahamed Cell Genetics and Plant Biotechnology Laboratory (CGPBL), Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh
  • Mir Md Mahbubur Rahman Cell Genetics and Plant Biotechnology Laboratory (CGPBL), Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh
  • Sayeda Mahfuja Khatun Holding No-55, East Talaimari, Motihar, Rajshahi, Bangladesh.
  • A K M Mohiuddin Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh.
  • Abdullah Mohammad Shohael Cell Genetics and Plant Biotechnology Laboratory (CGPBL), Department of Biotechnology and Genetic Engineering, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh

DOI:

https://doi.org/10.3329/ptcb.v34i2.78830

Keywords:

Aromatic rice, Callus induction, Kalijira, Somatic embryogenesis, Tissue culture, Tulshimala

Abstract

An efficient regeneration protocol via somatic embryogenesis for two aromatic Indica rice varieties, Kalijira and Tulshimala was developed. Using mature, dehusked seeds, the process involved callus initiation, embryo development and plant regeneration involving MS medium accompanied with specific concentrations of the auxins 2,4-D and NAA, along with the cytokinin BAP. Callus induction was optimized at 2 mg/l 2,4-D, and 1 mg/l NAA, yielding initiation rates of 87% for Kalijira and 95% for Tulshimala. The medium supplemented with 2 mg/l 2,4-D supported robust embryogenic calli development. Mature embryos were successfully regenerated on media comprising 0.1 mg/l NAA and 2.5 mg/l BAP for Kalijira while 2 mg/l BAP for Tulshimala, with 35% of Kalijira calli forming multiple shoots and 65% rooting, on the other hand Tulshimala achieved 70% shoot formation and 100% rooting. Transplanted plantlets adapted well, showing high survival rates.

Plant Tissue Cult. & Biotech. 34(2): 153-164, 2024 (December)

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Published

2025-01-07

How to Cite

Afrin, M., Ahmed, S., Ahamed, T., Rahman, M. M. M., Khatun, S. M., Mohiuddin, A. K. M., & Shohael, A. M. (2025). Optimization of Plant Growth Regulators for Efficient Embryogenic Callus Induction and Subsequent Plant Regeneration in Two Indigenous Aromatic Rice Varieties of Bangladesh. Plant Tissue Culture and Biotechnology, 34(2), 153–164. https://doi.org/10.3329/ptcb.v34i2.78830

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