Isolation of mouse internal organs for molecular and histopathological studies
Keywords:Mouse, Organ isolation
Since autoimmune diseases dramatically affect function of various tissues and organs in vivo, each integral organ of mouse has significant value in order to analyze the effect of any pharmaceuticals or test drugs to confirm their protective efficacy. Hence, this study reports step-by-step visual demonstration of isolation of various internal tissue organs such as liver, spleen, adipose tissue, muscles, and pancreas from mouse model which have significant contribution to design in-depth molecular level studies along with histomorphological evidences that may assist research to set innovative results at molecular level, thus may help to prove the efficacy of any test sample as a new drug of pharmaceutical importance.
Video Clip of Methodology:
Bajpai VK, Rather I, Nam GJ. Experimental strategy of animal trial for the approval of antidiabetic agents prior to their use in pre-human clinical trials. Bangladesh J Pharmacol. 2016; 11: 30-34.
Deng Y, Zhang Y, Lu Y, Z Y, Ren H. Hepatotoxicity and nephrotoxicity induced by the chlorpyrifos and chlorpyrifos-methyl metabolite, 3,5,6-trichloro-2-pyridinol, in orally exposed mice. Sci Tot Environ. 2016; 544: 507-14.
Haenen S, Clynen E, Nemery B, Hoet PHM, Vanoirbeek JAJ. Biomarker discovery in asthma and COPD: Application of proteomics techniques in human and mice. Eupa Open Proteom. 2014; 4: 101-12.
How to Cite
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).