Hydroxychloroquine induces inhibition of collagen type II and oligomeric matrix protein COMP expression in chondrocytes
The aim of this study was to investigate the effect of hydroxychloroquine on the level of collagen type II and oligomeric matrix protein COMP expression in chondrocytes of knee osteoarthritis. The rate of growth in cartilage cells was analyzed using MTT assay whereas the Col-2 and COMP expression levels were detected by RT-PCR and Western blotting analyses. For the determination of MMP-13 expression, ELISA test was used. The results revealed no significant change in the rate of cartilage cell proliferation in hydroxychloroquine-treated compared to untreated cells. Hydroxychloro-quine treatment exhibited concentration- and time-dependent effect on the inhibition of collagen type II and COMP expression in chondrocytes. However, its treatment caused a significant enhancement in the expression levels of MMP-13 compared to the untreated cells. Therefore, hydroxychloro-quine promotes expression of MMP-13 and reduces collagen type II and COMP expression levels in chondrocytes without any significant change in the growth of cells.
Ashfaq UA, Javed T, Rehman S, Nawaz Z, RiazuddinS. Lysosomotropic agents as HCV entry inhibitors. Virol J. 2011; 8: 163-68.
Brondello JM, Philipot D, Djouad F, Jorgensen C, Noël D. Cellular senescence is a common characteristic shared by preneoplasic and osteo-arthritic tissue. Open Rheumatol J. 2010; 4: 10-14.
Cawston T, Billington C, Cleaver C, Elliott S, Hui W, Koshy P, Shingleton B, Rowan A. The regulation of MMPs and TIMPs in cartilage turnover. Ann NY Acad Sci. 1999; 878: 120-29.
Costedoat-Chalumeau N, Leroux G, Piette J-P, AmouraZ. Why all systemic lupus erythematosus patients should begiven hydroxychloroquine treatment? Joint Bone Spine. 2010; 77: 4-5.
Eyre D. Collagen of articular cartilage. Arthritis Res. 2002; 4: 30-35.
Gentili C, Cancedda R. Cartilage and bone extracellular matrix. Curr Pharm Des 2009; 15: 1334-48.
Guzman-Morales J, Lafantaisie-Favreau CH, Chen G, Hoemann CD. Subchondral chitosan/blood implant-guided bone plate resorption and woven bone repair is coupled to hyaline cartilage regeneration from microdrill holes in aged rabbit knees. Osteoarthritis Cartilage. 2014; 22: 323-33.
Goldring MB. The role of the chondrocyte in osteoarthritis. Arthritis Rheum. 2000; 43: 1916-26.
Kim HA, Song YW. Apoptotic chondrocyte death in rheumatoid arthritis. Arthritis Rheum. 1999; 42: 1528-37.
Korth C, May BCH, Cohen FE, Prusiner SB. Acridine and phenothiazine derivatives as pharmacotherapeutics for prion disease. Proc Natl Acad Sci USA. 2001; 98, 9836-41.
Lo WC, Chen WH, Lin TC, Hwang SM, Zeng R, Hsu WC, Chiang YM, Liu MC, Williams DF, Deng WP. Preferential therapy for osteoarthritis by cord blood MSCs through regulation of chondrogenic cytokines. Biomaterials 2013; 34: 4739-48.
Mahoney E, Maddocks K, Flynn J, Jones J, Cole SL, Zhang X, Byrd JC, Johnson AJ. Identification of endoplasmicreticulum stress-inducing agents by antagonizing autophagy: A new potential strategy for identification of anti-cancer therapeutics in b-cell malignancies. Leuk Lymphoma. 2013; 54: 2685-92.
Roughley PJ. Articular cartilage and changes in arthritis: Non-collagenous proteins and proteoglycans in the extracellular matrix of cartilage. Arthritis Res. 2001; 3: 342-47.
Suarez-Almazor ME, Belseck E, SheaB, Homi, J, Wells G, Tugwell P. Antimalarials for treating rheumatoid arthritis. Cochrane Database Syst Rev. 2010; 4: CD000959.
Vasquez-Martin A, Lopez-Bonetc E, Cuti S, Oliveras-Ferraros C, Del Barco S, Martin-Castillo B, Menendez JA. Repositioning chloroquine and metformin to eliminate cancer stem cell traits in pre-malignant lesions. Drug Resist Updates. 2011; 14: 212-23.
Turajane T, Thitiset T, Honsawek S, Chaveewanakorn U, Aojanepong J, Papadopoulos KI. Assessment of chondrogenic differentiation potential of autologous activated peripheral blood stem cells on human early osteoarthritic cancelloustibial bone scaffold. Musculoskelet Surg. 2014; 98: 35-43.
Wattanachai T, Yonemitsu I, Kaneko S, Soma K. Functional lateral shift of the mandible effects on the expression of ECM in rat temporomandibular cartilage. Angle Orthod. 2009; 79: 652-59.
Xu M, Zhang L, Zhao L, Gao S, Han R, Su D, Lei G. Phosphorylation of osteopontin in osteoarthritis degenerative cartilage and its effect on matrix metalloprotease 13. Rheumatol Int. 2013; 33: 1313-19.
Copyright (c) 2016 Tao Li, Hong-Yan Shi, Yong-Xin Hua, Chen Gao, Qing Xia, Guang Yang, Bin Li
This work is licensed under a Creative Commons Attribution 4.0 International License.Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).