An Improved Plant Regeneration of Vanilla planifolia Andrews

Authors

  • Boon Chin Tan School of Biosciences, Faculty of Science, The University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan
  • Chiew Foan Chin School of Biosciences, Faculty of Science, The University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan
  • Peter Alderson School of Biosciences, Faculty of Science, The University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan

DOI:

https://doi.org/10.3329/ptcb.v21i1.9560

Keywords:

Regeneration, coconut water, nodal culture, vanilla

Abstract

Multiple shoots of Vanilla planifolia were induced from nodal explants under influence of different concentrations of plant growth regulators and coconut water (CW). A comparison of shoot regeneration between semi solid and liquid culture media was also investigated.  Ninety seven per cent of the explants produced a mean number of 9.6 shoots with a mean shoot length of 4.70 cm when cultured on liquid MS supplemented with 1.0 mg/l BAP in combination of 15% CW. Shoots generated were rooted with frequency of 93% on MS supplemented with 1.0 mg/l NAA with a mean number of 2.9 roots per shoot and mean length of 4.0 cm within four weeks. The plantlets were transferred to sand : compost mixture (1 : 2) with 85.0% survival rate recorded after four weeks of acclimation.

 

Key words: Regeneration, coconut water, nodal culture, vanilla

 

D.O.I. 10.3329/ptcb.v21i1.9560

 

Plant Tissue Cult. & Biotech. 21(1): 27-33, 2011 (June)

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Published

2012-01-24

How to Cite

Tan, B. C., Chin, C. F., & Alderson, P. (2012). An Improved Plant Regeneration of Vanilla planifolia Andrews. Plant Tissue Culture and Biotechnology, 21(1), 27–33. https://doi.org/10.3329/ptcb.v21i1.9560

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