In vitro Mass Propagation Protocol for Lobelia chinensis Lour.: Optimization of Growth Regulators and Acclimatization

Abstract

A reliable in vitro regeneration protocol has been developed for Lobelia chinensis Lour., an important medicinal plant with limited availability in Bangladesh. Nodal explants were cultured on MS medium with different cytokinins and auxins. The highest regeneration response (90%) was obtained on MS medium containing 5.55 µm/l BAP + 0.56 µm/l NAA, producing 14.00 ± 1.0 shoots per explant with a mean shoot length of 3.83 ± 0.17 cm within 28-42 days. In contrast, PGR-free MS medium showed only 20% response with 3.17 ± 0.44 shoots per explant and 1.17 ± 0.17 cm shoot length. The best rooting response (99%) and root per explant (29.33 ± 0.67) occurred in MS medium with 5.55 µm/l NAA within 14-21 days. While maximum root length (2.83 ± 0.17 cm) was observed in MS + 8.33 µm/l NAA. Regenerated plantlets were successfully acclimatized in a 1 : 1 mixture of garden soil and compost with 90% survival.

Plant Tissue Cult. & Biotech. 36(1): 57-65, 2026 (June)