Efficient Micropropagation and Genetic Fidelity Assessment of In vitro Raised Dragon Fruit (Hylocereus spp.) using ISSR Markers
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Plant Biotechnology Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka-1349, BangladeshAbstract
An efficient in vitro regeneration protocol was developed for two dragon fruit species (Hylocereus polyrhizus and Hylocereus undatus) widely cultivated in Bangladesh. The highest percentage (100%) of explants responded to shoot induction and maximum number of multiple shoots (12.4 ± 1.14 and 14.2 ± 1.14) and highest length of shoots (6.70 ± 1.50 and 5.70 ± 1.50 cm) were obtained in MS + 2.0 mg/l BAP + 0.5 mg/l NAA. The in vitro regenerated and proliferated shoots were transferred to ½ strength of MS with 0.8 mg/l NAA where 100% shoots were produced roots. The maximum number (7.5 ± 0.12 and 8.8 ± 0.4) and highest length (7.4 ± 0.3 and 5.8 ± 0.2 cm) of roots were found in this medium. After transplantation to soil the survival rate of in vitro regenerated plantlets was 100%. No ISSR polymorphism in the micropropagated plants was detected, while 3 Inter Simple Sequence Repeat (ISSR) markers were used in order to look for any somaclonal variation among in vitro-derived plants, demonstrating the high level of genetic fidelity in the plantlets produced by the regeneration technique reported in this work.
Plant Tissue Cult. & Biotech. 36(1): 37-47, 2026 (June)
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