A Half-diallel In vitro Androgenesis Study Using Drought- and Yield-Adapted Eggplant Parents

Authors

  • Md Samsuzzaman Department of Genetics and Plant Breeding, Gazipur Agricultural University, Gazipur-1706, Bangladesh
  • Nasrin Akter Ivy Department of Genetics and Plant Breeding, Gazipur Agricultural University, Gazipur-1706, Bangladesh
  • Md Ashraful Haque Institute of Biotechnology and Genetic Engineering, Gazipur Agricultural University, Gazipur-1706, Bangladesh
  • Emrul Kayesh Department of Horticulture, Gazipur Agricultural University, Gazipur-1706, Bangladesh
  • Md Ashraful Islam Department of Genetics and Plant Breeding, Gazipur Agricultural University, Gazipur-1706, Bangladesh
  • Mst Eshita Akter Department of Genetics and Plant Breeding, Gazipur Agricultural University, Gazipur-1706, Bangladesh
  • Sabuj Rahman Department of Genetics and Plant Breeding, Gazipur Agricultural University, Gazipur-1706, Bangladesh
  • Abu Shehab Bin Siddique Department of Genetics and Plant Breeding, Gazipur Agricultural University, Gazipur-1706, Bangladesh
  • Khondoker Afsara Tasnim Totine Department of Genetics and Plant Breeding, Gazipur Agricultural University, Gazipur-1706, Bangladesh
  • Shakil Ahamed Department of Genetics and Plant Breeding, Gazipur Agricultural University, Gazipur-1706, Bangladesh
  • Mehfuz Hasan Department of Genetics and Plant Breeding, Gazipur Agricultural University, Gazipur-1706, Bangladesh

DOI:

https://doi.org/10.3329/ptcb.v35i2.86678

Keywords:

Anther culture, Androgenic index, Embryo-like structure, Hybrid

Abstract

Anther culture-based doubled haploid (DH) technology offers an efficient approach for accelerating homozygosity in eggplant, although its application is often limited by strong genotype dependency and inconsistent regeneration. In this study, a set of F1 hybrids developed through a half-diallel mating design using drought-tolerant and high-yielding parental lines were evaluated for androgenic responsiveness under controlled in vitro conditions. Significant variation was observed among crosses for embryo-like structure (ELS) induction, green plantlet regeneration, and the composite Androgenic Index (AI). The diallel matrix revealed that several combinations, particularly RWE P11-2 × BP20-3, BP20-3 × RWE ©, B. Baria × BP20-3, and 3 (8) × BP20-3, showed superior ELS formation and regeneration efficiency, while albino production remained minimal across the population. Ranked AI values and Tukey groupings further identified RWE P11-2 × BP20-3 as the most promising cross for DH production. The results demonstrate strong parental effects on androgenesis, with BP20-3 and RWE P11-2 functioning as consistently responsive parents. Overall, this study highlights the potential of drought-adapted eggplant germplasm for efficient DH line development and provides valuable insights for integrating half-diallel-based selection with haploid breeding strategies.

Plant Tissue Cult. & Biotech. 35(2): 381-388, 2025 (December)

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Published

2026-01-04

How to Cite

Samsuzzaman, M., Ivy, N. A., Haque, M. A., Kayesh, E., Islam, M. A., Akter, M. E., … Hasan, M. (2026). A Half-diallel In vitro Androgenesis Study Using Drought- and Yield-Adapted Eggplant Parents. Plant Tissue Culture and Biotechnology, 35(2), 381–388. https://doi.org/10.3329/ptcb.v35i2.86678

Issue

Vol. 35 No. 2 (2025)

Section

Articles