A Half-diallel In vitro Androgenesis Study Using Drought- and Yield-Adapted Eggplant Parents

Authors

  • Md Samsuzzaman Department of Genetics and Plant Breeding, Gazipur Agricultural University, Gazipur-1706, Bangladesh
  • Nasrin Akter Ivy Department of Genetics and Plant Breeding, Gazipur Agricultural University, Gazipur-1706, Bangladesh
  • Md Ashraful Haque Institute of Biotechnology and Genetic Engineering, Gazipur Agricultural University, Gazipur-1706, Bangladesh
  • Emrul Kayesh Department of Horticulture, Gazipur Agricultural University, Gazipur-1706, Bangladesh
  • Md Ashraful Islam Department of Genetics and Plant Breeding, Gazipur Agricultural University, Gazipur-1706, Bangladesh
  • Mst Eshita Akter Department of Genetics and Plant Breeding, Gazipur Agricultural University, Gazipur-1706, Bangladesh
  • Sabuj Rahman Department of Genetics and Plant Breeding, Gazipur Agricultural University, Gazipur-1706, Bangladesh
  • Abu Shehab Bin Siddique Department of Genetics and Plant Breeding, Gazipur Agricultural University, Gazipur-1706, Bangladesh
  • Khondoker Afsara Tasnim Totine Department of Genetics and Plant Breeding, Gazipur Agricultural University, Gazipur-1706, Bangladesh
  • Shakil Ahamed Department of Genetics and Plant Breeding, Gazipur Agricultural University, Gazipur-1706, Bangladesh
  • Mehfuz Hasan Department of Genetics and Plant Breeding, Gazipur Agricultural University, Gazipur-1706, Bangladesh

Keywords:

Anther culture, Androgenic index, Embryo-like structure, Hybrid

Abstract

Anther culture-based doubled haploid (DH) technology offers an efficient approach for accelerating homozygosity in eggplant, although its application is often limited by strong genotype dependency and inconsistent regeneration. In this study, a set of F1 hybrids developed through a half-diallel mating design using drought-tolerant and high-yielding parental lines were evaluated for androgenic responsiveness under controlled in vitro conditions. Significant variation was observed among crosses for embryo-like structure (ELS) induction, green plantlet regeneration, and the composite Androgenic Index (AI). The diallel matrix revealed that several combinations, particularly RWE P11-2 × BP20-3, BP20-3 × RWE ©, B. Baria × BP20-3, and 3 (8) × BP20-3, showed superior ELS formation and regeneration efficiency, while albino production remained minimal across the population. Ranked AI values and Tukey groupings further identified RWE P11-2 × BP20-3 as the most promising cross for DH production. The results demonstrate strong parental effects on androgenesis, with BP20-3 and RWE P11-2 functioning as consistently responsive parents. Overall, this study highlights the potential of drought-adapted eggplant germplasm for efficient DH line development and provides valuable insights for integrating half-diallel-based selection with haploid breeding strategies.

Plant Tissue Cult. & Biotech. 35(2): 381-388, 2025 (December)

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Published

2026-01-04

How to Cite

Samsuzzaman, M., Ivy, N. A., Haque, M. A., Kayesh, E., Islam, M. A., Akter, M. E., Rahman, S., Siddique, A. S. B., Totine, K. A. T., Ahamed , S., & Hasan, M. (2026). A Half-diallel In vitro Androgenesis Study Using Drought- and Yield-Adapted Eggplant Parents. Plant Tissue Culture and Biotechnology, 35(2), 381-388. https://doi.org/10.3329/ptcb.v35i2.86678

Issue

Vol. 35 No. 2 (2025)

Section

Articles

How to Cite

Samsuzzaman, M., Ivy, N. A., Haque, M. A., Kayesh, E., Islam, M. A., Akter, M. E., Rahman, S., Siddique, A. S. B., Totine, K. A. T., Ahamed , S., & Hasan, M. (2026). A Half-diallel In vitro Androgenesis Study Using Drought- and Yield-Adapted Eggplant Parents. Plant Tissue Culture and Biotechnology, 35(2), 381-388. https://doi.org/10.3329/ptcb.v35i2.86678