In vitro Plant Regeneration from Different Leaf Segments of Verbesina encelioides and Correlation with Endogenous Level of IAA

Authors

  • Monika Karnawat School of Life Sciences, Jaipur National University, Jaipur, Rajasthan
  • Devendra Jain School of Life Sciences, Jaipur National University, Jaipur, Rajasthan
  • Abhijeet Singh School of Life Sciences, Jaipur National University, Jaipur, Rajasthan
  • C. P. Malik School of Life Sciences, Jaipur National University, Jaipur, Rajasthan

DOI:

https://doi.org/10.3329/ptcb.v20i2.6914

Keywords:

Plant regeneration, leaf segments, Verbesina encelioides

Abstract

Variable leaf segments of Verbesina encelioides exhibited different responses when cultured on MS supplemented with 0.5 mg/l BAP. With the addition of IAA/NAA to the basal medium completely inhibited the formation and growth of callus. Endogenous content of IAA in the different segments of the leaf when assayed revealed that middle segment  had highest level of IAA and apical and basal segments contained lowest contents. A specific correlation existed between the segment and callus formation.

 

Key words: Plant regeneration, leaf segments, Verbesina encelioides

 

D.O.I. 10.3329/ptcb.v20i2.6914

 

Plant Tissue Cult. & Biotech. 20(2): 195-201, 2010 (December)

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How to Cite

Karnawat, M., Jain, D., Singh, A., & Malik, C. P. (2011). In vitro Plant Regeneration from Different Leaf Segments of Verbesina encelioides and Correlation with Endogenous Level of IAA. Plant Tissue Culture and Biotechnology, 20(2), 195–201. https://doi.org/10.3329/ptcb.v20i2.6914

Issue

Vol. 20 No. 2 (2010)

Section

Articles