Regeneration and Transformation via Agrobacterium tumefaciens of Echinacea purpurea L.

Authors

  • Moemen Hanafy Institute of Plant Genetics, Plant Biotechnology Department, Leibniz University Hannover, Herrenhauser Str. 2- 30419 Hannover
  • Usama I. Aly Plant Biotechnology Department, National Research Centre, El-Behooth Str., Dokki, Cairo
  • Mohamed A. Matter Plant Biotechnology Department, National Research Centre, El-Behooth Str., Dokki, Cairo

DOI:

https://doi.org/10.3329/ptcb.v20i2.6888

Keywords:

Echinacea purpurea, Regeneration, A. tumefaciens, Transformation

Abstract

Leaf explants of Echinacea purpurea L. taken from aseptically germinated seedlings were inoculated with A. tumefaciens strains EHA105, carrying a binary vector conferring herbicide resistant bar gene and fungal resistant chitinase gene. Glufosinate ammonium-resistant shoots were regenerated on a medium containing BAP and NAA at a concentration of 4.88 and 0.053 µM, respectively. A subsequent transfer of shoots to medium containing BAP was necessary for stem elongation and leaf development. Transgenic Echinacea plants carrying bar and chitinase genes were selected for their resistance to glufosinate ammonium herbicide. Molecular analysis using PCR confirmed the integration of the transgenes into plant genome. This is the first report on genetic transformation of Echinacea plant using bar gene as a selectable marker.

Key words: Echinacea purpurea; Regeneration; A. tumefaciens; Transformation

D.O.I. 10.3329/ptcb.v20i2.6888

Plant Tissue Cult. & Biotech. 20(2): 101-111, 2010 (December)

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How to Cite

Hanafy, M., Aly, U. I., & Matter, M. A. (2011). Regeneration and Transformation via Agrobacterium tumefaciens of Echinacea purpurea L. Plant Tissue Culture and Biotechnology, 20(2), 101–111. https://doi.org/10.3329/ptcb.v20i2.6888

Issue

Vol. 20 No. 2 (2010)

Section

Articles