Development of In vitro Mass Propagation Protocol for Gerbera (Gerbera jamesonii Bolus) var. Orange

Authors

  • Nasrin Akter Department of Genetic Engineering and Biotechnology, Jashore University of Science and Technology, Jashore 7408, Bangladesh
  • Selim Sarkar Department of Genetic Engineering and Biotechnology, Jashore University of Science and Technology, Jashore 7408, Bangladesh
  • Naimul Hasan Department of Genetic Engineering and Biotechnology, Jashore University of Science and Technology, Jashore 7408, Bangladesh
  • Shaikh Mizanur Rahman Department of Genetic Engineering and Biotechnology, Jashore University of Science and Technology, Jashore 7408, Bangladesh
  • Md Abdur Rauf Sarkar Department of Genetic Engineering and Biotechnology, Jashore University of Science and Technology, Jashore 7408, Bangladesh

DOI:

https://doi.org/10.3329/ptcb.v32i2.63555

Keywords:

Mass propagation, Gerbera, Flower bud, Hormone-free rooting, Acclimatization

Abstract

An in vitro mass propagation protocol was developed for Gerbera (Gerbera jamesonii Bolus) var. orange using flower bud explants. The young (7 days) explants were found to be suitable for culture and highest (100%) callus induction was obtained in MS medium supplemented with 5.0 mg/l BAP with 1.0 mg/l NAA. The maximum shoot regeneration (95%) and the number of shoots (10) were observed in MS medium supplemented with 6.0 mg/l BAP with 0.3 mg/l NAA. The highest shoot multiplication frequency (95%) and the number of shoots (60) were obtained in MS medium supplemented with only 2.0 mg/l BAP. The well-developed highest number of roots (5.0) per plant and average root length (5.0 cm) with 100% root induction efficiency without callusing was recorded in the half strength of MS medium devoid of any hormonal supplement. Acclimatized in vitro grown plantlets were successfully transferred into the field condition.

Plant Tissue Cult. & Biotech. 32(2): 217-226, 2022 (December)

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Published

2022-12-29

How to Cite

Akter, N., Sarkar, S. ., Hasan, N. ., Rahman, S. M. ., & Rauf Sarkar, M. A. . (2022). Development of In vitro Mass Propagation Protocol for Gerbera (Gerbera jamesonii Bolus) var. Orange. Plant Tissue Culture and Biotechnology, 32(2), 217–226. https://doi.org/10.3329/ptcb.v32i2.63555

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