In vitro Flowering and Production of Seeds in Oxalis corniculata L. - an Important Medicinal Plant

Authors

  • VN Swetha Prasuna Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam-517425, Andhra Pradesh, India
  • CM Narendra Reddy Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam-517425, Andhra Pradesh, India
  • M Rajagopal Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam-517425, Andhra Pradesh, India
  • G Sudhasri Purna Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam-517425, Andhra Pradesh, India
  • B Srinivas Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam-517425, Andhra Pradesh, India

DOI:

https://doi.org/10.3329/ptcb.v32i2.63553

Keywords:

Plant growth regulators, In vitro flowering, Oxalis corniculata, production of seeds, medicinal plant.

Abstract

The multiple shoots (14.2) from Oxalis corniculata differentiated through bud breaking on MS medium with 2.0 mg/l BAP. The regenerated shoots were further sub-cultured on MS medium containing 1.0 mg/l BAP and 0.5 mg/l NAA. Vegetative to reproductive phase transition occurred in the cultures affected by plant growth regulators (PGRs), sucrose concentrations, strength of MS salts and light. Flower buds initiated from the regenerated shoots on half strength of MS salts with 1.0 mg/l Kn + 0.5 mg/l NAA and 3% sucrose. These flower buds opened under low light (10-15 μmol m-2 s-1) condition with 18h/6h photoperiod within 3 weeks of culture initiation. The shoots did not flower in the dark. Though smaller in size, the in vitro generated flowers were morphologically comparable to the natural flowers. Fruits were set in the cultures within 5 weeks.

Plant Tissue Cult. & Biotech. 32(2): 193-203, 2022 (December)

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Published

2022-12-29

How to Cite

Prasuna, V. S. ., Reddy, C. N. ., Rajagopal, M., Purna, G. S. ., & Srinivas, B. (2022). In vitro Flowering and Production of Seeds in Oxalis corniculata L. - an Important Medicinal Plant. Plant Tissue Culture and Biotechnology, 32(2), 193–203. https://doi.org/10.3329/ptcb.v32i2.63553

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