In vitro Propagation of Vanda testacea (Lindl.) Reichb. F, a Medicinally Important Threatened Orchid

Authors

  • Saranjeet Kaur Faculty, University Institute of Biotechnology, Division Biosciences, Chandigarh University, District- Mohali, Punjab, India

DOI:

https://doi.org/10.3329/ptcb.v31i2.57343

Keywords:

Asymbiotic, Orthodox seeds, Protocorms, Orchids, Growth regulators.

Abstract

The present study was planned to enable in vitro conservation of Vanda testacea, a highly medicinal orchid species through in vitro asymbiotic seed germination technique in Mitra orchid medium supplemented with cytokinins (Kn - 4.65 μM, BAP - 4.44 μM), and auxin (NAA- 5.37 μM). The germination frequency and initiation of germination was higher in NAA augmented medium and seedlings developed in 12.50 ± 0.50 weeks. Coconut water (20%) proved optimum for the multiplication of protocorm like bodies. Activated charcoal successfully checked the release of brownish exudates in the cultures.

Plant Tissue Cult. & Biotech. 31(2): 153-160, 2021 (December)

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Published

2022-01-10

How to Cite

Kaur, S. (2022). In vitro Propagation of Vanda testacea (Lindl.) Reichb. F, a Medicinally Important Threatened Orchid. Plant Tissue Culture and Biotechnology, 31(2), 153–160. https://doi.org/10.3329/ptcb.v31i2.57343

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Articles