In vitro Propagation of Bitter Gourd (Momordica charantia L.) from Nodal and Root Segments

Authors

  • M.A.Z. Al Munsur Department of Biotechnology, Bangladesh Agricultural University, Mymensingh
  • M.S. Haque Department of Biotechnology, Bangladesh Agricultural University, Mymensingh
  • K.M. Nasiruddin Department of Biotechnology, Bangladesh Agricultural University, Mymensingh
  • M.S. Hossain Department of Biotechnology, Bangladesh Agricultural University, Mymensingh

DOI:

https://doi.org/10.3329/ptcb.v19i1.4916

Keywords:

In vitro propagation, bitter gourd, nodal segments, Root segments

Abstract

Explants of nodal and root segments of bitter gourd were cultured on MS supplemented with various concentrations of BAP in combination with either 2,4-D or NAA. Nodal segments produced the highest percentage (93.75) of callus in MS supplemented with 1.0 mg/l 2,4-D and 1.0 mg/l BAP whereas, root segments produced the highest (85.00%) callus in 0.6 mg/l NAA and 2.5 mg/l BAP combination. A combination of 1.0 mg/l 2,4-D and 1.0 mg/l BAP exhibited 75.00% shoot regeneration from nodal segments. The highest shoot length (5.15 cm) was recorded with 2.5 mg/l BAP and 0.2 mg/l IAA from nodal segments. No sign of regeneration of shoot was found from root segments in any of the combinations. However, some combinations produced only roots.

 

Key words: In vitro propagation, bitter gourd, nodal segments, Root segments

 

D.O.I. 10.3329/ptcb.v19i1.4916

 

Plant Tissue Cult. & Biotech. 19(1): 45-52, 2009 (June)

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How to Cite

Munsur, M. A., Haque, M., Nasiruddin, K., & Hossain, M. (2010). In vitro Propagation of Bitter Gourd (Momordica charantia L.) from Nodal and Root Segments. Plant Tissue Culture and Biotechnology, 19(1), 45–52. https://doi.org/10.3329/ptcb.v19i1.4916

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