Agrobacterium-mediated Transformation and Constitutive Expression of PgNHX1 from Pennisetum glaucum L. in Oryza sativa L. cv. Binnatoa

Authors

  • S.M. Touhidul Islam Department of Biochemistry and Molecular Biology, University of Dhaka
  • R.S. Tammi 1Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar
  • Sneh L. Singla-Pareek International Centre for Genetic Engineering and Biotechnology, New Delhi
  • Z.I. Seraj Department of Biochemistry and Molecular Biology, University of Dhaka

DOI:

https://doi.org/10.3329/ptcb.v19i1.4912

Keywords:

Salinity tolerance, PgNHX1, CaMV35S, Binnatoa

Abstract

In an effort to improve salinity tolerance in rice, the Pennisetum glaucum vacuolar Na+/H+ antiporter gene (PgNHX1) was transformed and expressed in Bangla-deshi rice Binnatoa under the constitutive promoter CaMV35S. Transgenic status of the plant was confirmed by PCR and Southern blot hybridization. RT-PCR and Western dot-blot analyses were performed to validate the expression of the transgene at RNA and protein levels, respectively. At 160 mM (ECe of 16 dS/m) NaCl stress the transgenic seedlings showed enhanced tolerance compared to controls.

 

Key words:  Salinity tolerance, PgNHX1, CaMV35S, Binnatoa

 

D.O.I.10.3329/ptcb.v19i1.4912

 

Plant Tissue Cult. & Biotech. 19(1): 25-33, 2009 (June)

 

 

 

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How to Cite

Islam, S. T., Tammi, R., Singla-Pareek, S. L., & Seraj, Z. (2010). Agrobacterium-mediated Transformation and Constitutive Expression of PgNHX1 from Pennisetum glaucum L. in Oryza sativa L. cv. Binnatoa. Plant Tissue Culture and Biotechnology, 19(1), 25–33. https://doi.org/10.3329/ptcb.v19i1.4912

Issue

Vol. 19 No. 1 (2009)

Section

Articles