Tissue Culture Applications of Artemisia annua L. Callus for Indirect Organogenesis and Production Phytochemical

Authors

  • Ely Zayova Department of Plant-Soil Interactions, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia-1113, Bulgaria
  • Trendafil Nedev Department of Plant-Soil Interactions, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Sofia-1113, Bulgaria
  • Detelina Petrova Department of Plant Physiology, Faculty of Biology of the Sofia University “St. Kliment Ohridski”, Sofia-1164, Bulgaria
  • Miroslava Zhiponova Department of Plant Physiology, Faculty of Biology of the Sofia University “St. Kliment Ohridski”, Sofia-1164, Bulgaria
  • Venеta Kapchina Department of Plant Physiology, Faculty of Biology of the Sofia University “St. Kliment Ohridski”, Sofia-1164, Bulgaria
  • Ganka Chaneva Department of Plant Physiology, Faculty of Biology of the Sofia University “St. Kliment Ohridski”, Sofia-1164, Bulgaria

DOI:

https://doi.org/10.3329/ptcb.v30i1.47795

Keywords:

Artemisia annua, Indirect organogenesis, Phytochemical, Antioxidant activity

Abstract

Artemisia annua L. axenic plants were used to induce callogeneisis for production of phenolics and plant regeneration. Up to 95% callogenesis from leaf and stem explants on MS supplemented with 2.0 mg/l NAA or 2,4-D + 0.2 mg/l BAP (MSC1 and MSC2) was observed. Lower callus frequency but with improved embryogenic potential was observed upon subculture on medium with reduced auxin and increased BAP concentration (0.5 mg/l NAA or 2,4-D + 0.5 mg/l BAP) (MSC3 and MSC4). The leafinduced callus on NAA/BAP (MSC3) showed best antioxidant potential. Induced shoot regeneration occurred upon high concentration BAP combined with NAA rather than with 2,4-D (0.25 mg/l NAA or 2,4-D + 1.0 mg/l BAP, MSR 1 and MSR2, respectively). Optimal shoot multiplication and rooting were obtained on 1.0 mg/l BAP and 0.1 mg/l IBA, respectively, followed by acclimatization of regenerants to greenhouse conditions. This work aims at establishing protocol for A. annua preservation and biosynthesis of natural products.

Plant Tissue Cult. & Biotech. 30(1): 97-106, 2020 (June)

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Published

2020-06-25

How to Cite

Zayova, E., Nedev, T., Petrova, D., Zhiponova, M., Kapchina, V., & Chaneva, G. (2020). Tissue Culture Applications of Artemisia annua L. Callus for Indirect Organogenesis and Production Phytochemical. Plant Tissue Culture and Biotechnology, 30(1), 97–106. https://doi.org/10.3329/ptcb.v30i1.47795

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Articles