In vitro Propagation of Two Grapevine (Vitis vinifera L.) Cultivars under Conditions of Salt Stress

Authors

  • Getachew Kassa Institute of Biotechnology, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia
  • Tileye Feyissa Microbial, Cellular and Molecular Biology Department, College of Natural Sciences, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia

DOI:

https://doi.org/10.3329/ptcb.v30i1.47790

Keywords:

Canonannon, Chenine Blanc, Hyperhydricity, Salt tolerance

Abstract

This study was aimed to investigate salt tolerance of two grapevine cultivars, ‘Chenine Blanc’ and ‘Canonannon’ through in vitro propagation on medium containing different concentrations of NaCl. Single-node shoots were cultured on MS with 1.0 mg/l BAP in combination with 0.1 mg/l IBA and containing 0.25, 0.50, 0.75, 1.00 or 1.50% NaCl. NaCl free medium was used as control. Shoots of both cultivars were cultured on the same MS containing 0.25, 0.50, 0.75 or 1.00% CaCl2 to reduce hyperhydricity. The shoots were transferred to rooting medium followed by acclimatization in greenhouse. Number and length of shoots and roots, number of leaves and nodes, length of nodes, fresh and dry weight of roots and shoots decreased significantly in consistent trend as the concentration of NaCl increased. ‘Canonannon’ cultivar was found to be significantly more tolerant to NaCl than ‘Chenine Blanc’ in all parameters. The lowest percentage of hyperhydric shoots were obtained on medium containing 0.25% CaCl2. Therefore, ‘Canonannon’ cultivar can be planted in relatively saline soils as it is more tolerant to salt than ‘Chenine Blanc’.

Plant Tissue Cult. & Biotech. 30(1): 47-56, 2020 (June)

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Published

2020-06-25

How to Cite

Kassa, G., & Feyissa, T. (2020). In vitro Propagation of Two Grapevine (Vitis vinifera L.) Cultivars under Conditions of Salt Stress. Plant Tissue Culture and Biotechnology, 30(1), 47–56. https://doi.org/10.3329/ptcb.v30i1.47790

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