Conventional and Molecular Identification of Culturable Airborne Bacteria

Authors

  • Rezuana Naznin Department of Botany, Jagannath University, Dhaka-1100, Bangladesh
  • Nasrin Sultana Department of Botany, Jagannath University, Dhaka-1100, Bangladesh
  • Md Nur Hossain Bangladesh Council of Scientific and Industrial Research, Dhaka-1205, Bangladesh. 2Department of Botany, Dhaka University, Dhaka-1000, Bangladesh
  • Mohammad Nurul Islam Department of Botany, Dhaka University, Dhaka-1000, Bangladesh
  • Anika Tabassum Department of Botany, Jagannath University, Dhaka-1100, Bangladesh
  • Md Ataul Gani Department of Botany, Jagannath University, Dhaka-1100, Bangladesh
  • Mahbubah Jannat Department of Botany, Dhaka University, Dhaka-1000, Bangladesh

DOI:

https://doi.org/10.3329/ptcb.v30i1.47787

Keywords:

Atmospheric bacteria, 16S rRNA gene, Diversity

Abstract

Bacteria isolated from the environment during the present study were representative of normal microflora of the skin, respiratory and urinary tracts; it also includes some soil and water-borne pathogenic and nonpathogenic genera. Six samples from different locations were studied for bacterial investigation. Among 14 isolates obtained, 13 were Gram positive, and the rest one was Gram negative. Out of 13 Gram positive isolates, 12 were round-shaped non spore forming and were identified as Planococcus citreus, Stomatococcus mucilaginosus, Kocuria kristinae, Micrococcus agilis (2), Kytococcus sedentarius (2), Micrococcus luteus, Micrococcus lylae and M. roseus, Staphylococcus aureus, Staphylococcus epidermidis and rod-shaped non spore forming identified as Renibacterium salmoninarum. The Gram-negative bacteria was identified as Pseudomonas aeruginosa. Other than provisional identification, two isolates (JG 40 and SG 49) were further confirmed through molecular characterization on the basis of 16Sr RNA gene sequence analysis as Staphylococcus aureus and Pseudomonas aeruginosa repectively. Spearman’s correlation showed that air temperature and wind speed negatively correlated with the bacterial abundance. It is clear that none of the samples containing airborne pathogens collected was safe for human health due to presence of potentially pathogenic microorganisms. Many were human pathogenic as well as food poisoning microorganisms.

Plant Tissue Cult. & Biotech. 30(1): 15-25, 2020 (June)

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Published

2020-06-25

How to Cite

Naznin, R., Sultana, N., Hossain, M. N., Islam, M. N., Tabassum, A., Gani, M. A., & Jannat, M. (2020). Conventional and Molecular Identification of Culturable Airborne Bacteria. Plant Tissue Culture and Biotechnology, 30(1), 15–25. https://doi.org/10.3329/ptcb.v30i1.47787

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