In Vitro Micropropagation of Stevia rebaudiana Bertoni

Authors

  • Sabina Yesmin Plant Biotechnology Division, National Institute of Biotechnology, Ganakbari, Ashulia, Savar, Dhaka-1349, Bangladesh

DOI:

https://doi.org/10.3329/ptcb.v29i2.44516

Keywords:

Stevia rebaudiana, Nodal segments, Shoot tips, Micropropagation, Acclimatization

Abstract

Multiple shoots were obtained from both shoot tips and nodal segments cultured on MS fortified with different concentrations of BAP and Kn singly or in combination with low concentration of NAA. Maximum number of shoots (9.28 ± 0.61) were found on MS supplemented with 1.5 mg/l BAP and 05 mg/l NAA. In vitro regenerated shoots were separated and transferred onto half and full-strength MS supplemented with different concentration of IBA, IAA and NAA for root induction. Full strength MS containing 0.2 mg/l IBA was found to be best for root induction where 93.33% shoots were rooted. In vitro regenerated plants grew normally without showing any morphological variation and flowered after 40 days of transplantation.

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Published

2019-12-02

How to Cite

Yesmin, S. (2019). In Vitro Micropropagation of Stevia rebaudiana Bertoni. Plant Tissue Culture and Biotechnology, 29(2), 277–284. https://doi.org/10.3329/ptcb.v29i2.44516

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Articles