Improvement of Wax Oil Content of Embryonic Callus of Jojoba Using Gamma Radiation

Authors

  • Hattem M. El-Shabrawi Plant Biotechnology Department, National Research Centre (NRC), 12622 -El-Buhouth St., Dokki, Cairo, Egypt
  • Hend E. Wahba Medecinal and Aromatic Plant Department, National Research Centre (NRC), 12622-El-Buhouth St., Dokki, Cairo, Egypt
  • Ahmed M. Gabr Plant Biotechnology Department, National Research Centre (NRC), 12622 -El-Buhouth St., Dokki, Cairo, Egypt
  • Shafik I. El-Morsy Department of Botany, Faculty of Agriculture, Al-Azhar University, Cairo, 11884, Egypt
  • Mohamed A. Saber Department of Botany, Faculty of Agriculture, Al-Azhar University, Cairo, 11884, Egypt
  • Shawky A. Bekheet Plant Biotechnology Department, National Research Centre (NRC), 12622 -El-Buhouth St., Dokki, Cairo, Egypt

DOI:

https://doi.org/10.3329/ptcb.v29i2.44509

Keywords:

Embryonic callus, Jojoba, Gamma radiation, Wax oil content

Abstract

Embryogenic callus was obtained from the leaf explants of jojoba (Simmondsia chinensis (Link) Schneider cultured on MS containing 0.5 mg/l NAA and 0.5 mg/l Kn. Growth of embryonic callus increased in 0.5 mg/l Kn + 6% sucrose. In order to improve oil content, the embryonic callus was exposed to different doses (5, 10 and 15 Kr) of gamma radiation. It was found that oil content of embryonic callus of jojoba increased 1.41% by exposing to 5 Kr gamma radiation. Also, size of oil bodies in embryonic callus irradiated with 5 Kr increased compared to control. Furthermore, production of the fatty acids was enhanced by gamma irradiation. The highest percentage of total fixed oil (1.41) was recorded in 5 Kr radiation. However, the highest content of total fatty acids (98.612) was obtained when embryonic callus were exposed to 10 Kr of gamma radiation.

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Published

2019-12-02

How to Cite

El-Shabrawi, H. M., Wahba, H. E., Gabr, A. M., El-Morsy, S. I., Saber, M. A., & Bekheet, S. A. (2019). Improvement of Wax Oil Content of Embryonic Callus of Jojoba Using Gamma Radiation. Plant Tissue Culture and Biotechnology, 29(2), 207–217. https://doi.org/10.3329/ptcb.v29i2.44509

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Articles