Musa sp. paradisiaca Cultivar Gopi and Clonal fidelity Assessment using ISSR Marker

Authors

  • Rabindra Kumar Sinha Cytogenetics and Plant Biotechnology Laboratory, Department of Botany, Tripura University, Suryamaninagar-799022, Tripura, India
  • Puja Rani Saha Cytogenetics and Plant Biotechnology Laboratory, Department of Botany, Tripura University, Suryamaninagar-799022, Tripura, India
  • Bibhash Nath Cytogenetics and Plant Biotechnology Laboratory, Department of Botany, Tripura University, Suryamaninagar-799022, Tripura, India
  • H. Reshmi Singha Cytogenetics and Plant Biotechnology Laboratory, Department of Botany, Tripura University, Suryamaninagar-799022, Tripura, India
  • Anath Bandhu Das Department of Botany, Utkal University, Bhubaneswar, Odisha, India
  • Satyanarayan Jena Genetics and Molecular Biology, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India
  • Sangram Sinha Cytogenetics and Plant Biotechnology Laboratory, Department of Botany, Tripura University, Suryamaninagar-799022, Tripura, India

DOI:

https://doi.org/10.3329/ptcb.v29i2.44506

Keywords:

Musa, Cultivar Gopi, Micropropagation, Clonal fidelity, ISSR marker

Abstract

Attempt was made towards optimizing an in vitro shoot multiplication of banana cultivar Gopi with subsequent assessment of genetic stability. Experiments on enhanced shoot multiplication were conducted in MS fortified with BAP, Kn and 2-iP (4 mg/l) for a period of 8 weeks. Administration of exogenous root inducing growth regulator was eliminated to reduce culture investment and economy. Treatment with BAP supplemented medium proved to be optimum for shoot multiplication whereas 2-iP produced satisfactory results for shoot elongation. Long term incubation in all the treatments had been favorable for developing efficient root system. Hardening of in vitro grown plantlets showed high rate of survival (95%) upon transfer to potted soil. Assessment of clonal fidelity through inter simple sequence repeats (ISSR) analysis revealed 100% uniformity.

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Published

2019-12-02

How to Cite

Sinha, R. K., Saha, P. R., Nath, B., Singha, H. R., Das, A. B., Jena, S., & Sinha, S. (2019). Musa sp. paradisiaca Cultivar Gopi and Clonal fidelity Assessment using ISSR Marker. Plant Tissue Culture and Biotechnology, 29(2), 175–184. https://doi.org/10.3329/ptcb.v29i2.44506

Issue

Vol. 29 No. 2 (2019)

Section

Articles