In vitro Regeneration of Ginger (Zingiber officinale Roscoe)

Authors

  • M. Naimur Rahman Sumon Department of Botany, Jagannath University, Dhaka-1100, Bangladesh
  • Tanjina Akhtar Banu Plant Tissue Culture Section, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh
  • Sanjida Rahman Mollika Department of Botany, Jagannath University, Dhaka-1100, Bangladesh
  • Barna Goswami Plant Tissue Culture Section, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh
  • Mousona Islam Plant Tissue Culture Section, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh
  • Shahina Akter Plant Tissue Culture Section, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh
  • Ripa Akter Sharmin Department of Botany, Jagannath University, Dhaka-1100, Bangladesh
  • M. Salim Khan Plant Tissue Culture Section, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh

DOI:

https://doi.org/10.3329/ptcb.v29i2.44504

Keywords:

Regeneration, Zingiber officinale, Rhizome buds, Shoot tips

Abstract

An efficient and reproducible in vitro regeneration protocol was established for two varieties of ginger (Z. officinale Roscoe) namely, BARI Ada-1 and Chinese ginger accession number SG876). In case of BARI Ada-1 best result was obtained on MS supplemented with 2.0 mg/l BAP, 0.5 mg/l Kn and 0.5 mg/l NAA. In this combination, 95% rhizome bud explants responded within 6 - 8 days and mean number of shoots per explant was 8.79 ± 0.42. On the other hand, Chinese ginger showed best (90%) shoot regeneration response from the same explants on the same medium and hormonal combinations but in exchange of 0.25 mg/l NAA. In this hormonal composition shoot initiation started within 7 - 8 days of culture and mean number of shoots/explant was 6.83 ± 0.71 after 24 - 27 days of culture. Maximum root induction (90 and 80%) was found on MS supplemented with 0.5 mg/l IBA and 0.5 mg/l NAA in case of BARI Ada-1 and Chinese ginger, respectively. The in vitro regenerated plantlets were successfully transplanted into the soil after acclimatization.

Downloads

Download data is not yet available.
Abstract
490
PDF
439

Downloads

Published

2019-12-02

How to Cite

Sumon, M. N. R., Banu, T. A., Mollika, S. R., Goswami, B., Islam, M., Akter, S., Sharmin, R. A., & Khan, M. S. (2019). In vitro Regeneration of Ginger (Zingiber officinale Roscoe). Plant Tissue Culture and Biotechnology, 29(2), 151–159. https://doi.org/10.3329/ptcb.v29i2.44504

Issue

Section

Articles