In vitro Plant Regeneration in Cleopatra (Citrus reshni Hort. ex Tan.) by Direct Organogenesis

Authors

  • Urmila Devi School of Agricultural Biotechnology, Punjab Agricultural University, PAU, Ludhiana-141004, India
  • Harinder Singh Rattanpal Department of Fruit Science, Punjab Agricultural University, PAU, Ludhiana-141004, India

DOI:

https://doi.org/10.3329/ptcb.v28i2.39683

Keywords:

Shoot regeneration, Citrus reshni, Direct regeneration

Abstract

Maximum shoot regeneration (87.85%) and number of shoots per explant in the epicotyl segments having longitudinal cut in Citrus reshni var. Cleopatra were obtained on MT medium supplemented with BAP (2 mg/l) + NAA (0.2 mg/l). Maximum shoot length (5.10 cm), average leaf number per shoot (12), leaf length (2.12 cm) and width (1.5 cm) in Cleopatra were obtained on MT medium supplemented with BAP (2 mg/l) + NAA (0.2 mg/l). The maximum in vitro rooting (78.88 %) in the excised shoots occurred in liquid medium on MS supplemented with BAP (0.5 mg/l) + NAA (0.5 mg/l).

Plant Tissue Cult. & Biotech. 28(2): 251-260, 2018 (December)

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Published

2018-12-05

How to Cite

Devi, U., & Rattanpal, H. S. (2018). In vitro Plant Regeneration in Cleopatra (Citrus reshni Hort. ex Tan.) by Direct Organogenesis. Plant Tissue Culture and Biotechnology, 28(2), 251–260. https://doi.org/10.3329/ptcb.v28i2.39683

Issue

Vol. 28 No. 2 (2018)

Section

Articles