Elicitation of Bacoside Content Using Plant Growth Regulators in Cell Suspension Culture of Bacopa monnieri (L.) Wettst.

Authors

  • AV Kharde MGM College of Agricultural Biotechnology, Gandheli, Aurangabad-431003, MS, India
  • SV Kore Dr. D. Y. Patil Biotechnology and Bioinformatics Institute, Dr. D. Y. Patil Vidyapeeth, Pune-411033, MS, India
  • MB Khetmalas Rajiv Gandhi Institute of Information Technology and Biotechnology, Bharati Vidyapeeth Deemed University, Pune - 411046, MS, India

DOI:

https://doi.org/10.3329/ptcb.v28i2.39678

Keywords:

Bacopa monnieri, Bacoside, Callus culture, Suspension, Elicitation

Abstract

Cell suspension culture is used for the optimization of plant growth regulators used for the elicitation of bacoside content of Bacopa monnieri (L.) Wettst. Callus induction was carried out on MS supplemented with 2, 4-D (0.5 mg/l) by using leaf explants. For suspension culture MS liquid medium was supplemented with constant α-naphthalene acetic acid (0.5 mg/l) and casein hydrolysate (1 g/l) as well as the varied concentrations of 6-benzyleadenine, kinetin, thidiazuaron and chitosan. It was observed that bacoside production was increased by 6- benzyleadenine (0.5 mg/l), Kn (0.5 mg/l) and thidiazuaron (0.25 mg/l) was 3.07, 3.79 and 3.97 mg/g, respectively. This indicates that lower concentration of growth regulator favor the bacoside enhancement under in vitro conditions, while chitosan affects the bacoside production in suspension culture. In this study thidiazuaron at 0.25 mg/l induces maximum elicitation of bacoside content i.e. 3.97 mg/g.

Plant Tissue Cult. & Biotech. 28(2): 191-199, 2018 (December)

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Published

2018-12-05

How to Cite

Kharde, A., Kore, S., & Khetmalas, M. (2018). Elicitation of Bacoside Content Using Plant Growth Regulators in Cell Suspension Culture of Bacopa monnieri (L.) Wettst. Plant Tissue Culture and Biotechnology, 28(2), 191–199. https://doi.org/10.3329/ptcb.v28i2.39678

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