In vitro Regeneration and Over Expression of Pea DNA Helicase 45 (PDH45) Gene into the Local Cultivars of Chickpea (Cicer arietinum L.) through Agrobacteriummediated Genetic Transformation

Authors

  • Nuram Mubina Department of Botany, Barisal University, Barisal
  • MI Hoque Plant Breeding and Biotechnology Laboratory, Department of Botany, University of Dhaka, Dhaka?1000
  • RH Sarker Plant Breeding and Biotechnology Laboratory, Department of Botany, University of Dhaka, Dhaka?1000

DOI:

https://doi.org/10.3329/ptcb.v28i1.37204

Keywords:

Chickpea, In vitro regeneration, Genetic transformation, Salinity tolerance, PDH45 gene

Abstract

In vitro regeneration studies compatible to Agrobacterium-mediated genetic transformation were carried out using two different types of zygotic embryo derived explants namely, decapitated embryo (DE) and decapitated embryo with single cotyledon disc (DEC) from three varieties of chickpea (Cicer arietinum L.) such as BARI chhola-4, -5 and -9 cultivated in Bangladesh. The best responses towards in vitro shoot regeneration was obtained from decapitated embryo with DEC on MS containing 0.5 mg/l BAP, 0.5 mg/l Kn and 0.2 mg/l NAA. Healthy and effective roots from the regenerated shoots were developed on MS supplemented with 0.2 mg/l IBA. Genetic transformation was carried out with Agrobacterium strain LBA4404 containing the binary plasmid pCAMBIA1301- PDH45 to integrate salt tolerant PDH45 gene in locally grown varieties of chickpea. The transformed plantlets were successfully established in soil following adequate hardening. Integration of salt tolerant PDH45 gene within the genomic DNA was confirmed through GUS histochemical assay and PCR analysis.

Plant Tissue Cult. & Biotech. 28(1): 125-140, 2018 (June)

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Published

2018-06-28

How to Cite

Mubina, N., Hoque, M., & Sarker, R. (2018). In vitro Regeneration and Over Expression of Pea DNA Helicase 45 (PDH45) Gene into the Local Cultivars of Chickpea (Cicer arietinum L.) through Agrobacteriummediated Genetic Transformation. Plant Tissue Culture and Biotechnology, 28(1), 125–140. https://doi.org/10.3329/ptcb.v28i1.37204

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