Over Expression of p5cs Gene to Increase Cold Stress Tolerance in Iranian Species of Petunia

Authors

  • Maryam Jamshidnia Department of Plant Breeding and Biotechnology, Sari Agricultural Sciences and Natural Resources University (SANRU), Sari
  • Cyrus Ghobadi Department of Agricultural Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan 84156-83111
  • Badraldin Ebrahim Sayed Tabatabaei Department of Agricultural Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan 84156-83111
  • Ahad Yamchi Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan

DOI:

https://doi.org/10.3329/ptcb.v28i1.37196

Keywords:

Over expression, p5cs gene, Cold stress, Petunia sp.

Abstract

In order to examine the relationship between proline and cold stress in Iranian (local) genotype of petunia, p5cs gene was transferred to petunia through Agrobacterium-mediated transformation. The leaf discs from four weeks and shoot apices from 7-day-old petunia in vitro plants were co-cultivated with Agrobacterium strain LBA4404 harboring a plasmid pBI121 as the vector system for transformation of petunia. pBI121 plasmid containing β-glucuronidase (gus) gene as a reporter gene and Δ1-pyrroline-5-carboxylate synthetase (p5cs) gene and neomycin phosphotransferase (nptII) gene were used as a selectable marker. The co-cultivated leaf discs and shoot apices were transferred to the selective medium and thereafter to proliferation medium respectively. To confirm transformation, regenerated plants were subjected to the polymerase chain reaction (PCR), GUS histochemical and proline assays. The results confirmed the presence of the gus and p5cs gene in the genome of all transformants and the transformed plants were more tolerant (674.87 μg/g fresh leaves) than the wild types under stress conditions.

Plant Tissue Cult. & Biotech. 28(1): 35-44, 2018 (June)

Downloads

Download data is not yet available.
Abstract
895
PDF
845

Downloads

Published

2018-06-28

How to Cite

Jamshidnia, M., Ghobadi, C., Tabatabaei, B. E. S., & Yamchi, A. (2018). Over Expression of p5cs Gene to Increase Cold Stress Tolerance in Iranian Species of Petunia. Plant Tissue Culture and Biotechnology, 28(1), 35–44. https://doi.org/10.3329/ptcb.v28i1.37196

Issue

Section

Articles