High Frequency In vitro Regeneration of Gynura procumbens (Lour.) Merr.

Authors

  • Tanjina Akhtar Banu Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka?1205
  • Barna Goswami Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka?1205
  • Shahina Akter Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka?1205
  • Mousona Islam Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka?1205
  • Tammana Tanjin Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka?1205
  • Ahashan Habib Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka?1205
  • Salim Khan Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka?1205

DOI:

https://doi.org/10.3329/ptcb.v27i2.35026

Keywords:

Gynura procumbens, High frequency, Regeneration

Abstract

An efficient rapid in vitro regeneration protocol was described from nodal segment, leaf and petiole explants. MS medium supplemented with 1.0 mg/l BAP and 0.5 mg/l IAA was found best for the multiple shoot formation from nodal segments. In this combination 99% explants produced multiple shoots and the average number of shoots per explants was 20.1 ± 1.96. For petiole and leaf explants best response was observed on MS supplemented with 2.0 mg/l BAP, 1 mg/l IAA and 0.5 mg/l Kn. Petiole explants produced highest mean number of shoots/explant (22.9 ± 1.728) among the three explants when the explants were cultured on MS with 2.0 mg/l BAP, 1 mg/l IAA and 0.5 mg/l Kn. The highest frequency of root induction (100%) and mean number of roots/plantlets (11.75) were obtained on MS. The rooted plantlets were transferred for hardening following acclimatization and finally were successfully established in the field.

Plant Tissue Cult. & Biotech. 27(2): 207-216, 2017 (December)

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Published

2017-12-27

How to Cite

Banu, T. A., Goswami, B., Akter, S., Islam, M., Tanjin, T., Habib, A., & Khan, S. (2017). High Frequency In vitro Regeneration of Gynura procumbens (Lour.) Merr. Plant Tissue Culture and Biotechnology, 27(2), 207–216. https://doi.org/10.3329/ptcb.v27i2.35026

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