In vitro Regeneration of Blepharispermum subsessile DC: An Endangered Medicinal Plant of Odisha, India using Cotyledon Explants

  • Pranati Nayak Taxonomy and Conservation Division, Regional Plant Resource Centre, Bhubaneswar? 751015, Odisha
  • Kalidass C Taxonomy and Conservation Division, Regional Plant Resource Centre, Bhubaneswar? 751015, Odisha
Keywords: Blepharispermum subsessile, Endangered, In vitro regeneration, Cotyledon

Abstract

Multiple shoots were induced on cotyledon explants of in vitro grown seedlings of Blepharispermum subsessile DC, cultured on MS medium supplemented with various combinations and concentrations of BAP, IBA and GA3. The highest regenerative response was observed on medium containing 2.5 mg/l BAP where shoot buds initiated after 12 days of inoculation and about 32 shoots were produced in 30 days time. Addition of GA3 played a key role in leaf expansion and elongation of shoot buds. Addition of the auxin IBA to the induction medium resulted in more callus proliferation rather than shoot bud induction. The elongated shoots were transferred to root induction medium consisting of half strength MS supplemented with IAA, NAA and IBA. Highest rooting response (90%) was recorded in ½ MS supplemented with 1.0 mg/l IAA. Acclimatized plants were maintained in polybags with garden soil for future reintroduction program to their natural habitat.

Plant Tissue Cult. & Biotech. 26(2): 255-266, 2016 (December)

Downloads

Download data is not yet available.
Abstract
1584
PDF
1000

Author Biography

Pranati Nayak, Taxonomy and Conservation Division, Regional Plant Resource Centre, Bhubaneswar? 751015, Odisha


Published
2016-12-10
How to Cite
Nayak, P., & C, K. (2016). In vitro Regeneration of Blepharispermum subsessile DC: An Endangered Medicinal Plant of Odisha, India using Cotyledon Explants. Plant Tissue Culture and Biotechnology, 26(2), 255-266. https://doi.org/10.3329/ptcb.v26i2.30575
Section
Articles