Somatic Embryogenesis from Anther, Whole Flower, and Leaf Explants of Some Grapevine Cultivars

Authors

  • Maryam Karimi Alavijeh Department of Horticulture Science, Faculty of Agriculture, University of Tehran, Karaj
  • Ali Ebadi Department of Horticulture Science, Faculty of Agriculture, University of Tehran, Karaj
  • Abdolkarim Zarei Department of Plant Biotechnology, Jahrom Universtiy, Jahrom
  • Mansour Omidi Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Tehran, Karaj

DOI:

https://doi.org/10.3329/ptcb.v26i2.30572

Keywords:

Cultivar, Embryogenic calli, Media, Vitis vinifera

Abstract

To investigate the influence of cultivar, medium, and explants on production of somatic embryogenic callus in grapevine (Vitis vinifera) an attempt was made. After callus production, calli were transferred into GS1CA medium for embryogenesis. In GS1CA medium, anther explant of Shahroodi cultivar showed the highest potential for production of embryogenic calli. Results showed that whole flower explants did not produce any embryogenic calli. In addition, leaf explants of Red?Sultanina and Flame Seedless were cultured on MS containing 1mg 2,4?D, 0.1 mg BA, 1 g/l casein hydrolisate, 20 g/l sucrose and 7 g/l agar were able to produce embryonic calli. After three months, calli were transferred to the MS with different concentrations of BA (1, 2 and 3.5 mg/l) and IAA (2, 5 and 15 mg/l). Results showed that among cultivars and different hormonal treatments, the medium containing 5 mg/l BA and 2 mg/l IAA induced maximum embryogenesis in Flame?Seedless calli.

Plant Tissue Cult. & Biotech. 26(2): 219-230, 2016 (December)

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Author Biography

Maryam Karimi Alavijeh, Department of Horticulture Science, Faculty of Agriculture, University of Tehran, Karaj



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Published

2016-12-10

How to Cite

Alavijeh, M. K., Ebadi, A., Zarei, A., & Omidi, M. (2016). Somatic Embryogenesis from Anther, Whole Flower, and Leaf Explants of Some Grapevine Cultivars. Plant Tissue Culture and Biotechnology, 26(2), 219–230. https://doi.org/10.3329/ptcb.v26i2.30572

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