Improvement of Flax Drought Tolerance Using Gene Transfer

Authors

  • Rasha Sabry Tawfik Department of Plant Biotechnology, National Research Center, Cairo
  • Abdelfattah Badr Department of Botany and Microbiology, Faculty of Science, Helwan University
  • Reda Sammour Department of Systematics Botany, Faculty of Science, Tanta University
  • Usama Ibrahim Department of Plant Biotechnology, National Research Center, Cairo
  • Mohamed Matter Department of Plant Biotechnology, National Research Center, Cairo
  • Mahmoud Sakr Department of Plant Biotechnology, National Research Center, Cairo

DOI:

https://doi.org/10.3329/ptcb.v26i2.30570

Keywords:

Improvement of flax, Drought tolerance, Gene transfer

Abstract

Attempt was made to produce transgenic cell lines of flax cv. Blanka tolerant to drought stress. Genetic transformation systems were used to incorporate the DREB2A gene, as the specific gene for drought stress tolerance. In biolistic transformation, hypocotyl segments were bombarded with DREB2A and GFP genes at particle flight distance of 9 cm and rupture disc pressure of 1300 psi. The expression of the gene was observed under a light microscope after 24 and 48 hrs. In Agrobacterium?mediated transformation, the hypocotyl segments were incubated overnight with Agrobacterium culture at five optical density OD600 i.e. 0.2, 0.4, 0.6, 0.8 and 1 for 30 min with occasional stirring. Later, the explants were transferred to a selection regeneration medium supplemented with 50 mg/dm3 hygromycin and 300 mg/dm3 cefotaxime and subcultured every two weeks on a new selection medium. Molecular analysis confirmed the expression of the target DREB2A gene in flax genome.

Plant Tissue Cult. & Biotech. 26(2): 197-207, 2016 (December

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Author Biography

Rasha Sabry Tawfik, Department of Plant Biotechnology, National Research Center, Cairo



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Published

2016-12-10

How to Cite

Tawfik, R. S., Badr, A., Sammour, R., Ibrahim, U., Matter, M., & Sakr, M. (2016). Improvement of Flax Drought Tolerance Using Gene Transfer. Plant Tissue Culture and Biotechnology, 26(2), 197–207. https://doi.org/10.3329/ptcb.v26i2.30570

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Articles