Direct Organogenesis of Glinus lotoides L. ? Antihelmintic Herb Using Nodal Segments

Authors

  • Shiferaw Teshome Department of Biotechnology, Wolaita Sodo University, P.O. Box 138, Sodo
  • Tileye Feyissa Department of Biotechnology, Wolaita Sodo University, P.O. Box 138, Sodo and Institute of Biotechnology, Addis Ababa University, P.O. Box 1176, Addis Ababa

DOI:

https://doi.org/10.3329/ptcb.v26i1.29774

Keywords:

Glinus lotoides, Kinetin, MS medium, Nodal segment

Abstract

Glinus lotoides L. is a valuable medicinal herb for the treatment of human and livestock ailments. However, it was listed under endangered plant species due to over utilization and lower seed viability. The purpose of this study has been to develop a micropropagation method from nodal segments of G. lotoides. MS containing various concentrations of Kn was used for culture induction. Best culture establishment (95%) and highest mean shoot number (2.45 ± 0.37) were achieved on a medium containing 1.5 mg/l Kn. Even though different concentrations of 6?benzyl aminopurine (BAP), Kn and ??naphthalene acetic acid (NAA) were utilized for shoot proliferation, the highest number of shoots per nodal segment (3.66 ± 0.61) was attained in the medium containing 1.0 mg/l BAP in combination with 0.2 mg/l NAA. Shoots were rooted on half strength MS containing IBA, IAA and NAA. In a medium containing 1.5 mg/l IBA, 5.80 ± 1.10 roots per shoot, 1.05 ± 0.10 cm root length and 100% root generation frequency were obtained. Plantlets were successfully established under greenhouse conditions with 94% survival rate and no aberrant plants were detected.

Plant Tissue Cult. & Biotech. 26(1): 131-141, 2016 (June)

Downloads

Download data is not yet available.
Abstract
968
PDF
945

Author Biography

Shiferaw Teshome, Department of Biotechnology, Wolaita Sodo University, P.O. Box 138, Sodo



Downloads

Published

2016-09-27

How to Cite

Teshome, S., & Feyissa, T. (2016). Direct Organogenesis of Glinus lotoides L. ? Antihelmintic Herb Using Nodal Segments. Plant Tissue Culture and Biotechnology, 26(1), 131–141. https://doi.org/10.3329/ptcb.v26i1.29774

Issue

Section

Articles