Influence of Plant Growth Regulators and Medium Strength on Micropropagation of the Biodiesel Plant, Jatropha curcus

Authors

  • Mohamed R Rady Department of Plant Biotechnology, Genetic Engineering and Biotechnology Division, National Research Center, El?Buhouth St., (P.O. Box 12622), Dokki, Cairo
  • El Sayed Department of Plant Biotechnology, Genetic Engineering and Biotechnology Division, National Research Center, El?Buhouth St., (P.O. Box 12622), Dokki, Cairo
  • M Mervat Department of Chemistry, Faculty of Science, Cairo University, Cairo
  • Mohamed El Sayed Department of Plant Biotechnology, Genetic Engineering and Biotechnology Division, National Research Center, El?Buhouth St., (P.O. Box 12622), Dokki, Cairo
  • Usama I Aly Department of Plant Biotechnology, Genetic Engineering and Biotechnology Division, National Research Center, El?Buhouth St., (P.O. Box 12622), Dokki, Cairo

DOI:

https://doi.org/10.3329/ptcb.v26i1.29770

Keywords:

Jatropha curcas, Medium strength, Micropropagation

Abstract

Leaf explants were cultured to evaluate the effect of different auxins and cytokinins and its concentrations; MS salts on micropropagation of the promising biodiesel Jatropha curcas plant under Egyptian conditions. Results showed that shoot initiated on 0.5 mg/l BA + 0.25 mg/l IBA. Multiplication and elongation were found to be the best using 0.5 mg/l BA in combination with 0.05 mg/l IBA. The multiple shoots were cultured on MS or half strength of MS supplemented with different concentrations of IAA and IBA for rooting phase. Half strength of MS containing 1.0 mg/l IAA was the best for rooting of micropropagated shoots. The rooted plantlets were acclimated in sand : peat?moss mixture (1 : 1) successfully.

Plant Tissue Cult. & Biotech. 26(1): 85-96, 2016 (June)

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Author Biography

Mohamed R Rady, Department of Plant Biotechnology, Genetic Engineering and Biotechnology Division, National Research Center, El?Buhouth St., (P.O. Box 12622), Dokki, Cairo



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Published

2016-09-27

How to Cite

Rady, M. R., Sayed, E., Mervat, M., El Sayed, M., & Aly, U. I. (2016). Influence of Plant Growth Regulators and Medium Strength on Micropropagation of the Biodiesel Plant, Jatropha curcus. Plant Tissue Culture and Biotechnology, 26(1), 85–96. https://doi.org/10.3329/ptcb.v26i1.29770

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