Optimization of Somatic Embryogenesis Protocol for Locally Adapted Eggplant (Solanum melongena L.) Varieties in Bangladesh

Authors

  • M Ashraful Habib Agriculture Research & Development, International Rice Research Institute, Dhaka
  • M Abdul Muktadir Grain Legume Breeding, Faculty of Agriculture and Environment, The University of Sydney, Centre for Carbon Water and Food (CCWF), 380 Werombi Road, Camden, Brownlow Hill, NSW 2570
  • M Rezwan Kabir Biotechnology Division, Bangladesh Agricultural Research Institute, Gazipur?1701
  • MA Khaleque Mian Department of Genetics and Plant Breeding, Bangabandhu Sheikh Mujibur Rahman Agricultural University, Gazipur?1706
  • MA Yousuf Akhond Biotechnology Division, Bangladesh Agricultural Research Institute, Gazipur?1701

DOI:

https://doi.org/10.3329/ptcb.v26i1.29769

Keywords:

Optimization, Somatic embryogenesis, Solanum melongena

Abstract

Five eggplant cultivars were cultured on MS supplemented with 0.5 mg/l 2,4?D, resulting in the development of embryogenic calli. When the calli were subcultured onto growth regulator free MS, BARI Begun?4 produced the highest number of embryos (64) per explant followed by BARI Begun?1 (57). Later both the superior varieties in terms of embryo production were cultured on MS having 0.5, 1.0, 1.5 and 2.0 mg/l 2,4?D along with a control. BARI Begun?4 produced the highest number of embryos (67) on MS supplemented with 1.0 mg/l 2,4?D which was closely followed by BARI Begun?1 (62). Regenerated plantlets were successfully established in soil following acclimation.

Plant Tissue Cult. & Biotech. 26(1): 77-83, 2016 (June)

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Author Biography

M Ashraful Habib, Agriculture Research & Development, International Rice Research Institute, Dhaka



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Published

2016-09-27

How to Cite

Habib, M. A., Muktadir, M. A., Kabir, M. R., Mian, M. K., & Akhond, M. Y. (2016). Optimization of Somatic Embryogenesis Protocol for Locally Adapted Eggplant (Solanum melongena L.) Varieties in Bangladesh. Plant Tissue Culture and Biotechnology, 26(1), 77–83. https://doi.org/10.3329/ptcb.v26i1.29769

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