Influence of Medium Component on In vitro Propagation of Thai’s Endangered Orchid: Bulbophyllum nipondhii Seidenf.

Authors

  • Wittaya Pakum Plant Tissue Culture Research Unit, Department of Biology, Faculty of Science, Naresuan University, Phitsanulok?65000
  • Santi Watthana Queen Sirikit Botanic Garden, P.O. Box 7, Mae Rim, Chiang Mai 50180 and School of Biology, Institute of Science, Suranaree University of Technology, 111 University Avenue, Suranaree Subdistrict, Muang District, Nakhon Ratchasima 30000
  • Kanok orn Srimuang Department of Biotechnology, School of Agriculture and Natural Resources, University of Phayao, Phayao 56000,
  • Anupan Kongbangkerd Plant Tissue Culture Research Unit, Department of Biology, Faculty of Science, Naresuan University, Phitsanulok?65000

DOI:

https://doi.org/10.3329/ptcb.v26i1.29765

Keywords:

Bulbophyllum nipondhii, Organic supplement, Seed germination, Propagation

Abstract

In vitro propagation of a rare orchid, Bulbophyllum nipondhii was carried out. Five different media were tested to find the suitable medium for seed germination and seedling development. The tangible results were obtained on VW medium. To assess the effect of pollination types on seed germination and seedlings development, seeds derived from different self?, cross? and open?pollination were examined. Open?pollinated seeds produced the best germination and the highest seedling development, followed by cross? and self? pollination. VW medium was supplemented with 0, 25, 50 or 75 g/l potato extract (PE) and 0, 50, 100, 150 or 200 ml/l coconut water (CW) to identify their most suitable concentration. PE (75 g/l) with 100 ml/l CW was found to be best combination.

Plant Tissue Cult. & Biotech. 26(1): 37-46, 2016 (June)

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Author Biography

Wittaya Pakum, Plant Tissue Culture Research Unit, Department of Biology, Faculty of Science, Naresuan University, Phitsanulok?65000



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Published

2016-09-27

How to Cite

Pakum, W., Watthana, S., Srimuang, K. orn, & Kongbangkerd, A. (2016). Influence of Medium Component on In vitro Propagation of Thai’s Endangered Orchid: Bulbophyllum nipondhii Seidenf. Plant Tissue Culture and Biotechnology, 26(1), 37–46. https://doi.org/10.3329/ptcb.v26i1.29765

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