Genetic Transformation of a Local Tomato (Solanum lycopersicum L.) Variety of Bangladesh

Authors

  • Pronabananda Das Department of Botany, University of Dhaka, Dhaka?1000
  • Aneesa Ansari Department of Botany, University of Dhaka, Dhaka?1000
  • Mohammad Nurul Islam Department of Botany, University of Dhaka, Dhaka?1000
  • RH Sarker Department of Botany, University of Dhaka, Dhaka?1000

DOI:

https://doi.org/10.3329/ptcb.v25i1.24128

Keywords:

Genetic transformation, Agrobacterium, BARI Tomato?8

Abstract

An in vitro regeneration and Agrobacterium?mediated genetic transformation protocol was optimized for a local tomato variety, BARI Tomato?8 using cotyledonary leaf and hypocotyls explants. The explants were treated with various growth regulators in MS at different concentrations and combinations. Highest number of multiple shoot induction was observed from both the explants cultured in MS supplemented with 8.88 ?M BAP and 0.57 ?M IAA. Half strength of MS supplemented with 1.14 ?M IAA was found to be the best for root induction from excised shoots. Agrobacterium mediated genetic transformation was carried using pBI121 plasmid harboring ??glucuronidase (GUS) reporter and nptII selectable marker genes. Transient GUS assay confirmed that both the explants pre?cultured for two days showed best transformation efficiency in bacterial suspension having optical density (OD) of 0.8 (at 600 nm) for 15 min and co?cultivation period of 3 days. The shoots regenerated from transformed cotyledonary leaf explants survived at 200 mg/l kanamycin selection. The presence of expected amplicon corresponding to the GUS gene was confirmed by PCR. This protocol paves a way for developing disease resistant tomato variety using target gene/s.

Plant Tissue Cult. & Biotech. 25(1): 87-97, 2015 (June)

Downloads

Download data is not yet available.
Abstract
497
PDF
230

Author Biography

Pronabananda Das, Department of Botany, University of Dhaka, Dhaka?1000



Downloads

Published

2015-07-09

How to Cite

Das, P., Ansari, A., Islam, M. N., & Sarker, R. (2015). Genetic Transformation of a Local Tomato (Solanum lycopersicum L.) Variety of Bangladesh. Plant Tissue Culture and Biotechnology, 25(1), 87–97. https://doi.org/10.3329/ptcb.v25i1.24128

Issue

Section

Articles