Effect of Silver Nitrate and Amino Acids on High Frequency Plants Regeneration in Barley (Hordeum vulgare L.)

Authors

  • Mozidul Haque Plant Genetic Engineering Laboratory, Institute of Biological Sciences, University of Rajshahi, Rajshahi?6205
  • Abu Baker Siddique Plant Genetic Engineering Laboratory, Institute of Biological Sciences, University of Rajshahi, Rajshahi?6205
  • SM Shahinul Islam Plant Genetic Engineering Laboratory, Institute of Biological Sciences, University of Rajshahi, Rajshahi?6205

DOI:

https://doi.org/10.3329/ptcb.v25i1.24124

Keywords:

Barley, Immature embryos, Somatic embryogenesis

Abstract

Three Bangladeshi barley genotypes viz. BARI barley?1, 3 and 6 were selected for this study to evaluate the efficiency of callus induction and plant regeneration. The effect of five doses of AgNO3 singly, and combined with amino acids (Lproline, L?glutamine) on callus induction and plant regeneration efficiency was evaluated using BARI barley?3 and 6. The maximum values of callus induction were recorded at 49.20 and 32.66% for BARI barley?6 and 3, respectively when 2.0 mg/l AgNO3 and 200 mg/l L?glutamine were added to the callus induction medium. Moreover, plant regeneration remarkably increased on MS + 1.0 mg/l BAP + 1.5 mg/l AgNO3 + 150 mg/l L?glutamine as 37.20% in BARI barley?6 and 16.13% in BARI barley?3. For rooting AgNO3 singly affect positively, whereas negative influence was observed in combinations with any amino acids. However, by using AgNO3 and amino acids, around < 4, < 27 and < 5 fold increase in callus induction were obtained. Regeneration and rooting were also found to increase considerably.

Plant Tissue Cult. & Biotech. 25(1): 37-50, 2015 (June)

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Author Biography

Mozidul Haque, Plant Genetic Engineering Laboratory, Institute of Biological Sciences, University of Rajshahi, Rajshahi?6205



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Published

2015-07-09

How to Cite

Haque, M., Siddique, A. B., & Islam, S. S. (2015). Effect of Silver Nitrate and Amino Acids on High Frequency Plants Regeneration in Barley (Hordeum vulgare L.). Plant Tissue Culture and Biotechnology, 25(1), 37–50. https://doi.org/10.3329/ptcb.v25i1.24124

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