Effect of Silver Nitrate and Amino Acids on High Frequency Plants Regeneration in Barley (Hordeum vulgare L.)

Abstract

Three Bangladeshi barley genotypes viz. BARI barley?1, 3 and 6 were selected for this study to evaluate the efficiency of callus induction and plant regeneration. The effect of five doses of AgNO₃ singly, and combined with amino acids (Lproline, L?glutamine) on callus induction and plant regeneration efficiency was evaluated using BARI barley?3 and 6. The maximum values of callus induction were recorded at 49.20 and 32.66% for BARI barley?6 and 3, respectively when 2.0 mg/l AgNO₃ and 200 mg/l L?glutamine were added to the callus induction medium. Moreover, plant regeneration remarkably increased on MS + 1.0 mg/l BAP + 1.5 mg/l AgNO₃ + 150 mg/l L?glutamine as 37.20% in BARI barley?6 and 16.13% in BARI barley?3. For rooting AgNO₃ singly affect positively, whereas negative influence was observed in combinations with any amino acids. However, by using AgNO₃ and amino acids, around < 4, < 27 and < 5 fold increase in callus induction were obtained. Regeneration and rooting were also found to increase considerably.

Plant Tissue Cult. & Biotech. 25(1): 37-50, 2015 (June)