Seed Germination and in vitro Plant Regeneration through Callus Culture of Two Lychnis Species

  • Kee Hwa Bae National Institute of Biological Resources, 42 Hwangyeong?ro, Seo?gu, Incheon, 404?708
  • Eui Soo Yoon Department of Biology, College of Nature Sciences, Kongju National University, Kongju 314?701
Keywords: Callus culture, Seed germination, Lychnis cognate, Lychnis fulgens, Regeneration

Abstract

Lychnis cognate Maxim and Lychnis fulgens Fish. Ex Spreng are two valued ornamental plants in Korea. Soaking of seeds in GA3 solution remarkably promoted germination up to 60%, but the control (0 mg/l) was not effective (> 5%). To select an adequate temperature for seed germination, seeds, previously soaked in a 1000 mg/l GA3 for 24 hrs, were incubated at 15, 20, 25, and 30°C. Seed germination of over 20% was obtained at 15, 20, and 25°C, but only 10% at 30°C. These results indicate that the seeds of L. cognate and L. fulgens are in a such dormant state that they hardly germinate even by dormancy breaker (GA3) and low (15 ? 25°C) temperature treatment. The highest callus induction was observed in the leaf explants of the seedlings on MS containing specific concentrations of 3.0 mg/l BA and 1.0 mg/l NAA. The adventitious shoot was formed < 90% of calli on 1/2 WPM medium. The height of in vitro propagated plantlet was no different media used for regeneration. This in vitro propagation protocol should be useful for conservation of endangered and ornamental plant.

Plant Tissue Cult. & Biotech. 25(1): 1-12, 2015 (June)

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Author Biography

Kee Hwa Bae, National Institute of Biological Resources, 42 Hwangyeong?ro, Seo?gu, Incheon, 404?708


Published
2015-07-09
How to Cite
Bae, K., & Yoon, E. (2015). Seed Germination and in vitro Plant Regeneration through Callus Culture of Two Lychnis Species. Plant Tissue Culture and Biotechnology, 25(1), 1-12. https://doi.org/10.3329/ptcb.v25i1.24110
Section
Articles