Grouping of Pseudomonas spp. isolated from dieback- affected sissoo (Dalbergia sissoo Roxb.) using phylogenetic analyses

Authors

  • HP Mühlbach Biocentre Klein Flottbek, University of Hamburg, Hamburg
  • H Tantau Biocentre Klein Flottbek, University of Hamburg, Hamburg
  • A Stubbe Biocentre Klein Flottbek, University of Hamburg, Hamburg
  • D Palm Biocentre Klein Flottbek, University of Hamburg, Hamburg
  • J Schulze Biocentre Klein Flottbek, University of Hamburg, Hamburg
  • MI Hoque Department of Botany, University of Dhaka, Dhaka?1000
  • RH Sarker Department of Botany, University of Dhaka, Dhaka?1000
  • Sk S Alam Department of Botany, University of Dhaka, Dhaka?1000
  • ML Saha Department of Botany, University of Dhaka, Dhaka?1000
  • Md S Khan BCSIR, Tissue Culture Section, Dhaka?1205

DOI:

https://doi.org/10.3329/ptcb.v24i2.23549

Keywords:

Grouping, Pseudomonus, Dieback?affected, Dalbergia sissoo, Phytogenetic analyses

Abstract

Dieback of sissoo (Dalbergia sissoo Roxb.) is a devastating plant disease, whose causative biotic agents were not yet identified unequivocally. Our previous studies revealed that bacteria belonging to the genus Pseudomonas were associated with dieback?affected sissoo trees. To study the bacterial community associated with dieback-affected sissoo trees, DNA based approaches were used in the present work. Sequencing almost the complete 16S rRNA gene of 33 selected Pseudomonas isolates allowed them to be grouped into two main clusters only. The major group consisted of 19 isolates related to P. oryzihabitans, while in the minor one ten isolates related to P. putida were found together with very few other pseudomonads. Almost the same clustering was obtained a with the independent grouping methods of amplified ribosomal DNA restriction analysis (ARDRA). These results strongly indicate that mainly bacteria strains related to Pseudomonas oryzihabitans were associated with dieback-affected Dalbergia sissoo trees. Immuno-histological studies with thin-sections of sissoo roots using Pseudomonas-specific antibodies showed that bacteria had invaded the root parenchyma.

Plant Tissue Cult. & Biotech. 24(2): 141-153, 2014 (December)

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Author Biography

HP Mühlbach, Biocentre Klein Flottbek, University of Hamburg, Hamburg



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Published

2015-06-02

How to Cite

Mühlbach, H., Tantau, H., Stubbe, A., Palm, D., Schulze, J., Hoque, M., Sarker, R., Alam, S. S., Saha, M., & Khan, M. S. (2015). Grouping of Pseudomonas spp. isolated from dieback- affected sissoo (Dalbergia sissoo Roxb.) using phylogenetic analyses. Plant Tissue Culture and Biotechnology, 24(2), 141–153. https://doi.org/10.3329/ptcb.v24i2.23549

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