Induction of Direct Adventitious Shoot Regeneration in Pear (Pyrus communis L.)

  • Mahdieh Yousefiara Department of Tissue Culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran (ABRII), Mahdasht Road, P.O. Box 31535-1897, Karaj
  • Maryam Jafarkhani Kermani Department of Tissue Culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran (ABRII), Mahdasht Road, P.O. Box 31535-1897, Karaj
  • Abdolreza Bagheri 1Department of Biotechnology and Plant Breeding, Faculty of Agriculture , Ferdowsi University of Mashhad, P. O. Box 91775-1163, Mashhad
  • Ali Akbar Habashi Department of Tissue Culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran (ABRII), Mahdasht Road, P.O. Box 31535-1897, Karaj
  • Hamid Abdollahi Department of Horticultural Research, Seed and Plant Improvement Institute, P.O. Box 4119, Karaj
Keywords: Induction, Direct shoot regeneration, Pyrus communis, Thidiazuron

Abstract

Efficient direct shoot regeneration of two pear (Pyrus communis L.), namely  'bartlett' and 'dargazi' was successfully developed for the use in future genetic engineering research. The basal MS and NN media supplemented with different concentrations of TDZ (0, 2.5, 5, 7.5 ?M) or BAP (0, 4, 8, 16 ?M) in combination with NAA (1 ?M) were compared. The result showed that direct adventitious shoot regeneration in pear was highly dependent on genotype, explant type and culture media. 'dargazi' had higher rate of regeneration compared to 'bartlett' and in both cultivars the highest per cent of regeneration was observed in lower sections of the leaves. Although the highest per cent of regeneration in 'bartlett' (38) was attained in the NN medium containing 2.5 µM TDZ and 1 µM NAA, but the differences in shoot regeneration between media containing 5 or 7.5 µM TDZ and 1 µM NAA were not significant. The highest per cent of regeneration in 'dargazi' (56) was obtained in NN medium containing 7.5 µm TDZ and 1 µm NAA.

Plant Tissue Cult. & Biotech. 24(1): 87-92, 2014 (June)

D. O. I. http://dx.doi.org/10.3329/ptcb.v24i1.19215

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Abstract
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Published
2014-06-22
How to Cite
Yousefiara, M., Kermani, M., Bagheri, A., Habashi, A. A., & Abdollahi, H. (2014). Induction of Direct Adventitious Shoot Regeneration in Pear (Pyrus communis L.). Plant Tissue Culture and Biotechnology, 24(1), 87-92. https://doi.org/10.3329/ptcb.v24i1.19215
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Articles