Effects of Hormonal and Basal Nutrient Medium on In vitro Regeneration of an Ornamental Plant - Muscari armeniacum Leichtlin. ex Baker

  • Mustafa Abul Kalam Azad Department of Botany, University of Rajshahi, Rajshahi-6205
  • Muhammad Nurul Amin Department of Botany, University of Rajshahi, Rajshahi-6205
Keywords: Plant growth regulator, Micropropagation, Bulb-scale, Leaf-sheath, Grape hyacinth

Abstract

In vitro propagation system has been developed for an important ornamental and medicinal plant, Muscari armeniacum Leichtil. ex Bak. A range of a cytokinin and auxin concentration has been investigated for axillary bulblet proliferation, and direct and indirect adventitious bulblet regeneration from the explants whole bulb, one fourth part of bulb, bulb-scale of ex vitro (field grown mature bulb), and only leaf-sheath explants of in vitro grown bulblet. Axillary bulblet regeneration occurred on MS containing 2.0 - 8.0 ?M BAP or Kn. Direct adventitious bulblets were induced successfully on MS basal medium supplemented with various concentrations of BAP or Kn (1.0 - 4.0 ?M) in combi-nation of either NAA, IBA, or 2,4-D (0.5 - 4.0 ?M). The maximum frequency of adventitious bulblets regeneration occurred from both bulb-scale and leaf-sheath explants on MS with 4.0 ?M BAP and 2.0 ?M NAA, IBA, or 2,4-D. The highest frequency (95.5%) of indirect adventitious bulblets was obtained from in vitro grown leaf-sheathderived callus on MS containing 4.0 ?M BAP with 1.0 ?M 2,4-D whereas, highest number (80.2) and average length (55.5 cm) of bulblets were obtained on MS supplemented with 4.0 ?M BAP and 1.0 ?M NAA. In vitro grown bullets was rooted successfully on MS with 0.5 - 4.0 ?M of IBA, NAA, or IAA. The rooted bulblets were transferred to garden soil and successfully established under ex vitro environment.

DOI: http://dx.doi.org/10.3329/ptcb.v22i2.14200

Plant Tissue Cult. & Biotech. 22(2): 113-126, 2012 (December)

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Abstract
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Published
2013-03-19
How to Cite
Azad, M. A., & Amin, M. N. (2013). Effects of Hormonal and Basal Nutrient Medium on In vitro Regeneration of an Ornamental Plant - Muscari armeniacum Leichtlin. ex Baker. Plant Tissue Culture and Biotechnology, 22(2), 113-126. https://doi.org/10.3329/ptcb.v22i2.14200
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Articles