Micropropagation of <i>Vanasushava pedata</i> - An Endangered Medicinal Plant of South India

Authors

  • S Karuppusamy Department of Botany, Sri Krishnadevaraya University, Andhra Pradesh-515 003, India
  • C Kiranmai Department of Botany, Sri Krishnadevaraya University, Andhra Pradesh-515 003, India
  • V Aruna Department of Botany, Sri Krishnadevaraya University, Andhra Pradesh-515 003, India
  • T Pullaiah Department of Botany, Sri Krishnadevaraya University, Andhra Pradesh-515 003, India

DOI:

https://doi.org/10.3329/ptcb.v16i2.1109

Keywords:

Vanasushava pedata, Medicinal plant, Nodal explants, Micropropagation, Successful acclimation

Abstract

An efficient in vitro propagation of an endangered medicinal plant Vanasushava pedata (Apiaceae) by axillary shoot proliferation from nodal segments of mature plants was designed. The medium type and growth regulators markedly influenced in vitro regeneration of V. pedata. An in vitro plantlet production system has been investigated on MS with the synergistic combination of BA (5.0 mg/l), IAA (0.1 mg/l) and 3 % sucrose which promoted the maximum number of shoots (8.6) as well as enhanced shoot lengths. Subculturing of nodal segments from in vitro derived shoots on a similar medium enabled continuous production of healthy shoots with a similar frequency. Rooting was highest (100%) on half strength MS containing IAA (2.0 mg/l). Micropropagated plants established in garden soil and forest humus (1 : 1) were uniform and identical to the donor plants with respect of growth characteristics as well as floral features. These in vitro-raised plants grew normally in greenhouse and natural habitat without showing any morphological variation.  

Key words: Vanasushava pedata, Medicinal plant, Nodal explants, Micropropagation, Successful acclimation

DOI = 10.3329/ptcb.v16i2.1109

Plant Tissue Cult. & Biotech. 16(2): 85-94, 2006 (December)

Downloads

Download data is not yet available.
Abstract
1019
PDF
873

Downloads

How to Cite

Karuppusamy, S., Kiranmai, C., Aruna, V., & Pullaiah, T. (2008). Micropropagation of <i>Vanasushava pedata</i> - An Endangered Medicinal Plant of South India. Plant Tissue Culture and Biotechnology, 16(2), 85–94. https://doi.org/10.3329/ptcb.v16i2.1109

Issue

Section

Articles