In vitro Propagation of <i>Thuja occidentalis</i> Through Apical Shoot Culture

Authors

  • MH Kabir Plant Biotechnology and Genetic Engineering Division, IFRB, Atomic Energy Research Establishment, Ganakbari, Savar, Dhaka-1000, Bangladesh
  • PK Roy Plant Biotechnology and Genetic Engineering Division, IFRB, Atomic Energy Research Establishment, Ganakbari, Savar, Dhaka-1000, Bangladesh
  • Golam Ahmed Plant Biotechnology and Genetic Engineering Division, IFRB, Atomic Energy Research Establishment, Ganakbari, Savar, Dhaka-1000, Bangladesh

DOI:

https://doi.org/10.3329/ptcb.v16i1.1099

Keywords:

In vitro, Propagation, Thuja occidentalis, Apical shoot

Abstract

In vitro plant regeneration of Thuja occidentalis was obtained in apical shoot cultures from field grown plants. Hormone free MS medium 100% explants produced shoots. The average number of shoots per explant was 6.57 ± 0.45 and the average shoot length of 4.5 ± 0.27 cm were recorded in this medium. Shoots rooted well when they were transferred into half strength MS with 1.0 mg/l IBA. The average number of root per shoot was 3.92 ± 0.28 and the average root length of 3.64 ± 0.38 cm were observed in this medium. No morphological variants were observed during the passage of in vitro culture.

Key words: In vitro, Propagation, Thuja occidentalis, Apical shoot

DOI = 10.3329/ptcb.v16i1.1099

Plant Tissue Cult. & Biotech. 16(1): 5-9, 2006 (June)

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How to Cite

Kabir, M., Roy, P., & Ahmed, G. (2008). In vitro Propagation of <i>Thuja occidentalis</i> Through Apical Shoot Culture. Plant Tissue Culture and Biotechnology, 16(1), 5–9. https://doi.org/10.3329/ptcb.v16i1.1099

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