High Frequency Regeneration of Plantlets from Immature Male Floral Explants of Musa paradisica cv. Puttabale - AB Genome

Authors

  • K. Girish Kumar Department of P. G. Studies and Research in Biotechnology, Bioscience Block, Kuvempu University, Jnana Sahyadri, Shankaraghatta-577 451, Shivamogga (Dist.), Karnataka
  • V. Krishna Department of P. G. Studies and Research in Biotechnology, Bioscience Block, Kuvempu University, Jnana Sahyadri, Shankaraghatta-577 451, Shivamogga (Dist.), Karnataka
  • Venkatesh Venkatesh Department of P. G. Studies and Research in Biotechnology, Bioscience Block, Kuvempu University, Jnana Sahyadri, Shankaraghatta-577 451, Shivamogga (Dist.), Karnataka
  • K. Pradeep Department of P. G. Studies and Research in Biotechnology, Bioscience Block, Kuvempu University, Jnana Sahyadri, Shankaraghatta-577 451, Shivamogga (Dist.), Karnataka

DOI:

https://doi.org/10.3329/ptcb.v21i2.10243

Keywords:

Musa pardisica, Puttabale, Regeneration, Male floral explants

Abstract

High frequency in vitro regeneration for mass multiplication from immature male floral explants of Musa paradisica cv. Puttabale on MS supplemented with adenine sulfate (160 mg/l), tyrosine (100 mg/l), sucrose (40 g/l) and gelled with 0.8 g/l agar was attempted.  For callus induction the combinations of 2, 4-D and BAP were tested at 1.0 - 10.0 mg/l and 0.5 - 5.0 mg/l, respectively. For shoot bud formation combinations of BAP and TDZ were also tested at 1.0 - 5.0 mg/l and 0.1 - 0.5 mg/l, respectively.  Luxuriant proliferation and high frequency induction (97.0%) of  callus  was  noticed from the  accessory  floral  part  of  the  explant  at

7.0 mg/l 2, 4-D and 1.0 mg/l BAP, later it preceded towards the gynoecium. Interaction of BAP (2.0 5.0 mg/l) and TDZ (0.2 - 0.5 mg/l) would provoke high frequency shoot bud differentiation from the floral calli and a mean of 29.40 ± 6.10 shootlets per callus was obtained at 4 mg/l BAP and 0.4 mg/l TDZ. Rooting of the microshoots was achieved on MS containing 0.6 mg/l NAA and 0.2% activated charcoal.

 

 

Key words: Musa pardisica, Puttabale, Regeneration, Male floral explants.

 

D. O. I. 10.3329/ptcb.v21i2.10243

 

Plant Tissue Cult. & Biotech. 21(2): 199-205, 2011 (December)

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Published

2011-12-31

How to Cite

Kumar, K. G., Krishna, V., Venkatesh, V., & Pradeep, K. (2011). High Frequency Regeneration of Plantlets from Immature Male Floral Explants of Musa paradisica cv. Puttabale - AB Genome. Plant Tissue Culture and Biotechnology, 21(2), 199–205. https://doi.org/10.3329/ptcb.v21i2.10243

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