In vitro Propagation of Stemona tuberosa Lour. - A Rare Medicinal Plant through High Frequency Shoot Multiplication using Nodal Explants

Authors

  • Animesh Biswas Department of Botany, Government B. L. College, Khulna
  • M. A. Bari Institute of Biological Sciences, Rajshahi University, Rajshahi-6205
  • Mohashweta Roy Institute of Biological Sciences, Rajshahi University, Rajshahi-6205
  • S. K. Bhadra Department of Botany, University of Chittagong, Chittagong

DOI:

https://doi.org/10.3329/ptcb.v21i2.10238

Keywords:

Nodal explant, Stemona tuberose, Rare medicinal plant, Direct organogenesis

Abstract

Nodal segments of Stemona tuberosa Lour. were found to proliferated without any differentiation on to MS supplemented with 3.0 mg/l BAP and 0.5 mg/l NAA under continuous dark condition. After 20 days of inoculation under dark condition the cultures were transferred to a daily cycle of 16/8 hrs light/dark photoperiod and there the proliferated nodal segments underwent direct organogenesis producing huge number of shoot buds (25.87/culture). The shoot buds underwent rapid elongation on a range of BAP (0.1 - 1.5 mg/l) and IBA (0.1 - 1.0 mg/l) supplemented MS. Rooting of elongated shoot buds was successfully achieved (90%) in half strength MS with 1.0 mg/l NAA. The plantlets were finally established in outside environment through a successive phase of acclima-tization.

 

Key words: Nodal explant, Stemona tuberose, Rare medicinal plant, Direct organogenesis

 

D. O. I. 10.3329/ptcb.v21i2.10238

 

Plant Tissue Cult. & Biotech. 21(2): 151-159, 2011 (December)

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Published

2011-12-31

How to Cite

Biswas, A., Bari, M. A., Roy, M., & Bhadra, S. K. (2011). In vitro Propagation of Stemona tuberosa Lour. - A Rare Medicinal Plant through High Frequency Shoot Multiplication using Nodal Explants. Plant Tissue Culture and Biotechnology, 21(2), 151–159. https://doi.org/10.3329/ptcb.v21i2.10238

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