In vitro Shoot Proliferation and Plant Regeneration of Phlogacanthus thyrsiflorus Nees. a Rare Medicinal Shrub of Bangladesh

Authors

  • A.K. M. Sayeed Hassan Biological Research Division, BCSIR Laboratories, Dhaka-1205
  • Nadira Begum Biological Research Division, BCSIR Laboratories, Dhaka-1205
  • Rebeka Sultana Biological Research Division, BCSIR Laboratories, Dhaka-1205
  • Rahima Khatun Biological Research Division, BCSIR Laboratories, Dhaka-1205

DOI:

https://doi.org/10.3329/ptcb.v21i2.10236

Keywords:

Phlogacanthus thyrsiflorus, Shoot proliferation, Plant regeneration

Abstract

An efficient protocol was developed for shoot proliferation and plant regeneration of Phlogacanthus thyrsiflorus Nees. (Acanthaceae) - a rare medicinal shrub of Bangladesh, through in vitro culture using shoot tip and nodal explants. Best shoot induction was observed on MS with 1.0 mg/l BAP + 0.5 mg/l NAA, in which 84.2% of nodal explants responded to produce maximum number (12.4 ± 0.66) of shoots per culture. In vitro raised shoots rooted on half-strength MS with 0.5 mg/l IBA + 0.5 mg/l NAA. For acclimation and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%.

Key words: Phlogacanthus thyrsiflorus, Shoot proliferation, Plant regeneration

 

D. O. I. 10.3329/ptcb.v21i2.10236

 

Plant Tissue Cult. & Biotech. 21(2): 135-141, 2011 (December)

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Published

2011-12-31

How to Cite

Hassan, A. M. S., Begum, N., Sultana, R., & Khatun, R. (2011). In vitro Shoot Proliferation and Plant Regeneration of Phlogacanthus thyrsiflorus Nees. a Rare Medicinal Shrub of Bangladesh. Plant Tissue Culture and Biotechnology, 21(2), 135–141. https://doi.org/10.3329/ptcb.v21i2.10236

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