Prolactin gene diversity and its influence on milk yield traits in Ayrshire dairy cattle from Southern Russia

Authors

  • Nadezhda Vasilievna Shirokova Department of Biotechnology, Don State Agrarian University, Rostov-on-Don, Russian Federation. https://orcid.org/0000-0002-5607-3044
  • Vladimir Khristoforovich Fiodorov Department of Biology, Morphology and Virology, Don State Agrarian University, Rostov-on-Don, Russian Federation. https://orcid.org/0000-0002-7579-711X
  • Ivan Fiodorovich Gorlov Livestock Production Department, Povolzhsky Research Institute of Manufacture and Processing of Meat and Dairy Products, Volgograd, Russian Federation. https://orcid.org/0000-0002-8683-8159
  • Marina Ivanovna Slozhenkina Department of Livestock Products Storage and Processing, Povolzhsky Research Institute of Manufacture and Processing of Meat and Dairy Products, Volgograd, Russian Federation. https://orcid.org/0000-0001-9542-5893
  • Elena Yurievna Anisimova Farm Animal Breeding and Genetics Laboratory, Povolzhsky Research Institute of Manufacture and Processing of Meat and Dairy Products, Volgograd, Russian Federation. https://orcid.org/0000-0002-7508-3897
  • Natalia Ivanovna Mosolova Dairy Quality Control Laboratory, Povolzhsky Research Institute of Manufacture and Processing of Meat and Dairy Products, Volgograd, Russian Federation. https://orcid.org/0000-0001-6559-6595

Keywords:

Dairy cattle; Genetic diversity; Milk yield; PCR-RFLP; SNPs

Abstract

Objective: The objective of this research was to discover single-nucleotide variations in the prolactin (PRL) gene and to evaluate their correlation with milk production characteristics in Ayrshire cows reared in southern Russia. Materials and Methods: DNA (deoxyribonucleic acid) samples were extracted from the blood of cows (n = 300) using a commercial kit. The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was employed to identify and quantify genotypes and allele frequencies. Genetic indices were calculated, and statistical processing was performed. A general linear model was used to investigate the relationship between single-nucleotide polymorphisms and milk productivity in cows. Results: The distribution of the AA, AB, and BB genotypes in the studied population was 90%, 0%, and 10%, respectively. The genetic equilibrium in the Ayrshire cow population was preserved (χ² = 0.2879). The results indicate the presence of allelic diversity in the prolactin gene in this population. The milk yield per lactation in cows with the PRL_AA genotype was 7,378.50 kg, and for PRL_BB, it was 6,569.63 kg. Ayrshire cows with the PRL_BB genotype yielded less milk compared to their PRL_AA counterparts by 10.96% (p < 0.05). Conclusion: Using the obtained results in the future selection of offspring with desirable PRL genotypes at an earlier age could significantly increase the efficiency of breeding work in the studpulation and accelerate the process of creating a herd with higher productivity potential.

J. Adv. Vet. Anim. Res., 12(4): 1125–1131, December 2025

http://doi.org/10.5455/javar.2025.l973

Downloads

Download data is not yet available.
Abstract
13
PDF
10

Downloads

Published

2026-04-22

How to Cite

Shirokova, N. V., Fiodorov, V. K., Gorlov, I. F., Slozhenkina, M. I., Anisimova, E. Y., & Mosolova, N. I. (2026). Prolactin gene diversity and its influence on milk yield traits in Ayrshire dairy cattle from Southern Russia. Journal of Advanced Veterinary and Animal Research, 12(4), 1125–1131. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/88508

Issue

Vol. 12 No. 4 (2025)

Section

Original Articles

License

Copyright (c) 2025 Nadezhda Vasilievna Shirokova, Vladimir Khristoforovich Fiodorov, Ivan Fiodorovich Gorlov, Marina Ivanovna Slozhenkina, Elena Yurievna Anisimova, Natalia Ivanovna Mosolova

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

Authors who publish with this journal agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).