Dietary effects of organic selenium and zinc combination on in vitro microbial dynamics in the rumen
Keywords:
Microbial composition; selenium; trace mineral; ZincAbstract
Objective: This study used an in vitro approach to investigate the effects of combined organic selenium and zinc supplementation on rumen microbial communities. Materials and Methods: A completely randomized design was employed with five dietary treatments: basal diet only (CON), CON + selenium 0.30 ppm (part per million) + zinc 60 ppm (SZ-1), CON + selenium 0.45 ppm + zinc 60 ppm (SZ-2), CON + selenium 0.30 ppm + zinc 90 ppm (SZ-3), and CON + selenium 0.45 ppm + zinc 90 ppm (SZ-4). Selenium and zinc were provided in the form of organic-chelated methionine. Results: Analysis of rumen microbiota through 16S rRNA gene sequencing showed no significant differences in microbial diversity (p > 0.05); however, microbial composition was significantly affected. SZ-2, SZ-3, and SZ-4 groups exhibited an increased prevalence of Bacteroidetes and Proteobacteria and a reduction in Firmicutes compared to CON and SZ-1 (p < 0.05). The relative abundance of Patescibacteria and Euryarchaeota was also reduced in the SZ-2, SZ-3, and SZ-4 groups (p < 0.05). At the genus level, Prevotella, Ruminococcus, and Rikenellaceae RC9 gut groups were enriched in SZ-2, SZ-3, and SZ-4, whereas Lachnospiraceae XPB1014 and Christensenellaceae R-7 group decreased (p < 0.05). Conclusion: Combined selenium and zinc supplementation as organic trace minerals significantly modulates rumen microbial composition, enhancing the relative abundance of carbohydrate-degrading bacteria while reducing methanogen-related taxa.
J. Adv. Vet. Anim. Res., 12(3): 795–805, September 2025
80
111
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Moh Sofi’ul Anam, Budi Prasetyo Widyobroto, Andriyani Astuti, Gunawan Gunawan, Ali Agus
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).