Effects of supplementation of Bacillus spp. on blood metabolites, antioxidant status, and gene expression pattern of selective cytokines in growing Barki lambs

  • Sabry Mousa Department of Internal Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt
  • Ahmed Elsayed Department of Animal Health and Poultry, Desert Research Center (DRC), Matariya, Cairo, Egypt
  • Basma Marghani Department of Physiology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt
  • Ahmed Ateya Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Mansoura University, Mansoura, E
Keywords: Bacillus subtilis; immunity; antioxidant status; RT-PCR; sheep

Abstract

Objective: In this study, we investigated the potential immune-enhancing effects in addition to anti-oxidative stress properties of commercially accessible Bacillus subtilis supplementation in Barki lambs.

Materials and Methods: Twenty apparently healthy weaned Barki lambs were used in this study and distributed randomly into two experimental groups: Negative control group, received control basal diet without any feed supplements and a supplemented group, received control basal diet supplemented with water added to commercially accessible bacilli at 1 gm/l/day for 30 consecutive days. Blood samples were collected from each lamb before starting the experiment (T0), 2 weeks (T15), and 4 weeks (T30) post-supplementation for serum biochemical analyses, total leucocytes and lymphocytes count, and real-time polymerase chain reaction assays.

Results: The supplemented group showed a significant increase (p < 0.05) in the total number of leukocytes and the number of lymphocytes, lysozyme activity, reduced glutathione, total antioxidant capacity with a significantly lower malondialdehyde values at T30 and significantly higher levels (p < 0.05) of serum catalase and nitric oxide at T15 as compared with control ones. B. subtilis elicited maximal up-regulation of most of the studied genes compared with the control group.

Conclusion: The results herein suggest that B. subtilis could be used as useful nutritional supplements to support the immune system in healthy lambs.

J. Adv. Vet. Anim. Res., 6(3): 333-340, September 2019

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Published
2019-09-12
How to Cite
Mousa, S., Elsayed, A., Marghani, B., & Ateya, A. (2019). Effects of supplementation of Bacillus spp. on blood metabolites, antioxidant status, and gene expression pattern of selective cytokines in growing Barki lambs. Journal of Advanced Veterinary and Animal Research, 6(3), 333-340. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/43108
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Original Articles