Evaluation of LAMP for detection and/or screening of Leptospira spp. infection among domestic animals in the Philippines

  • Gabriel Alexis SP. Tubalinal
  • Michelle M. Balbin
  • Marvin A. Villanueva
  • Clarissa Yvonne J. Domingo
  • Claro N. Mingala

Abstract

Objective: This study assessed the applicability of loop-mediated isothermal amplification (LAMP) for the detection of leptospirosis among domesticated animals and sewage rats. Specifically, it evaluated the ability of LAMP to amplify Leptospira spp. targeting the 16s rRNA gene in boiled urine samples.

Materials and methods: A total of 140 samples from different domestic animals were tested for the presence of the antigen. A nested-polymerase chain reaction (nPCR) protocol was used to compare and determine the sensitivity of LAMP in detecting Leptospira spp. The LAMP was also evaluated by comparing its amplification result using agarose gel electrophoresis and color change using dye.

Results: Positivity rate of Leptospira spp. antigen was 29.0% (40/140) for LAMP and 9.3% (13/140) for nPCR. Also, LAMP results for gel electrophoresis and dye color change varied in some samples that may be due to the interpretation of the result in dye color change.

Conclusion: Overall, LAMP is a rapid, sensitive, and cost-effective diagnostic method compared with nPCR. Also, LAMP has a potential application as pen-side screening, surveillance, and clinical diagnostic kits of infectious diseases without requiring advance equipment and skilled personnel.

http://doi.org/10.5455/javar.2018.e299

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Author Biographies

Gabriel Alexis SP. Tubalinal

College of Veterinary Science and Medicine, Central Luzon State University, Nueva Ecija, Philippines

Michelle M. Balbin

Biosafety and Environment Section, Philippine Carabao Center National Headquarters and Gene Pool, Nueva Ecija, Philippines

Marvin A. Villanueva

Biosafety and Environment Section, Philippine Carabao Center National Headquarters and Gene Pool, Nueva Ecija, Philippines

Clarissa Yvonne J. Domingo

College of Veterinary Science and Medicine, Central Luzon State University, Nueva Ecija, Philippines

Claro N. Mingala

Biosafety and Environment Section, Philippine Carabao Center National Headquarters and Gene Pool, Nueva Ecija, Philippines

Department of Animal Science, College of Agriculture, Central Luzon State University, Nueva Ecija, Philippines

Published
2018-12-29
How to Cite
Tubalinal, G., Balbin, M., Villanueva, M., Domingo, C., & Mingala, C. (2018). Evaluation of LAMP for detection and/or screening of Leptospira spp. infection among domestic animals in the Philippines. Journal of Advanced Veterinary and Animal Research, 5(4), 459-465. Retrieved from https://www.banglajol.info/index.php/JAVAR/article/view/39512
Section
Original Articles